Development of in situ hybridization for detection of elephant endotheliotropic herpesvirus in Asian elephants

Development of in situ hybridization for detection of elephant endotheliotropic herpesvirus in Asian elephants

© 2018, 2018 The Author(s). Elephant endotheliotropic herpesvirus (EEHV) is one of the most important viral infectious diseases affecting the elephant population worldwide, especially juveniles and young adults. We developed a chromogenic in situ hybridization (ISH) test for detection of EEHV in Asi...

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Main Authors: Varankpicha Kochakul, Kittikorn Boonsri, Saruda Tiwananthagorn, Chalermchart Somgird, Chatchote Thitaram, Kidsadagon Pringproa
Format: Journal
Published: 2018
Subjects:
Veterinary
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85046781636&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/59178
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-591782018-09-05T04:40:57Z Development of in situ hybridization for detection of elephant endotheliotropic herpesvirus in Asian elephants Varankpicha Kochakul Kittikorn Boonsri Saruda Tiwananthagorn Chalermchart Somgird Chatchote Thitaram Kidsadagon Pringproa Veterinary © 2018, 2018 The Author(s). Elephant endotheliotropic herpesvirus (EEHV) is one of the most important viral infectious diseases affecting the elephant population worldwide, especially juveniles and young adults. We developed a chromogenic in situ hybridization (ISH) test for detection of EEHV in Asian elephants (Elephas maximus). Digoxigenin (DIG) DNA probes from the polymerase and terminase genes of EEHV were synthesized using a PCR DIG-labeling method, and detection of hybridized probe to target EEHV DNA was carried out by anti-DIG immunolabeling. Distribution of EEHV-1A and EEHV-4 genomes was found to be prominent in mononuclear phagocytic cells of spleen and endothelial cells of visceral organs. ISH enables the detection of EEHV infection and has applications in understanding pathogenesis of EEHV in Asian elephants. 2018-09-05T04:40:57Z 2018-09-05T04:40:57Z 2018-07-01 Journal 19434936 10406387 2-s2.0-85046781636 10.1177/1040638718773810 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85046781636&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/59178
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Veterinary
spellingShingle Veterinary
Varankpicha Kochakul
Kittikorn Boonsri
Saruda Tiwananthagorn
Chalermchart Somgird
Chatchote Thitaram
Kidsadagon Pringproa
Development of in situ hybridization for detection of elephant endotheliotropic herpesvirus in Asian elephants
description © 2018, 2018 The Author(s). Elephant endotheliotropic herpesvirus (EEHV) is one of the most important viral infectious diseases affecting the elephant population worldwide, especially juveniles and young adults. We developed a chromogenic in situ hybridization (ISH) test for detection of EEHV in Asian elephants (Elephas maximus). Digoxigenin (DIG) DNA probes from the polymerase and terminase genes of EEHV were synthesized using a PCR DIG-labeling method, and detection of hybridized probe to target EEHV DNA was carried out by anti-DIG immunolabeling. Distribution of EEHV-1A and EEHV-4 genomes was found to be prominent in mononuclear phagocytic cells of spleen and endothelial cells of visceral organs. ISH enables the detection of EEHV infection and has applications in understanding pathogenesis of EEHV in Asian elephants.
format Journal
author Varankpicha Kochakul
Kittikorn Boonsri
Saruda Tiwananthagorn
Chalermchart Somgird
Chatchote Thitaram
Kidsadagon Pringproa
author_facet Varankpicha Kochakul
Kittikorn Boonsri
Saruda Tiwananthagorn
Chalermchart Somgird
Chatchote Thitaram
Kidsadagon Pringproa
author_sort Varankpicha Kochakul
title Development of in situ hybridization for detection of elephant endotheliotropic herpesvirus in Asian elephants
title_short Development of in situ hybridization for detection of elephant endotheliotropic herpesvirus in Asian elephants
title_full Development of in situ hybridization for detection of elephant endotheliotropic herpesvirus in Asian elephants
title_fullStr Development of in situ hybridization for detection of elephant endotheliotropic herpesvirus in Asian elephants
title_full_unstemmed Development of in situ hybridization for detection of elephant endotheliotropic herpesvirus in Asian elephants
title_sort development of in situ hybridization for detection of elephant endotheliotropic herpesvirus in asian elephants
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85046781636&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/59178
_version_ 1681425203075219456

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