In vitro investigation of Mucuna pruriens seed extracts to treat erectile dysfunction

© 2018, Chiang Mai University. M. pruriens seeds are a natural product with health benefits, including treating erectile dysfunction (ED). However, the active compounds and their mechanisms of action are unclear. In this study, we used polarity gradient extraction to isolate four fractions from the...

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Main Authors: Natthachai Duangnin, Thanyaluck Phitak, Peraphan Pothacharoen, Prachya Kongtawelert
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/59203
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spelling th-cmuir.6653943832-592032018-09-05T04:41:20Z In vitro investigation of Mucuna pruriens seed extracts to treat erectile dysfunction Natthachai Duangnin Thanyaluck Phitak Peraphan Pothacharoen Prachya Kongtawelert Multidisciplinary © 2018, Chiang Mai University. M. pruriens seeds are a natural product with health benefits, including treating erectile dysfunction (ED). However, the active compounds and their mechanisms of action are unclear. In this study, we used polarity gradient extraction to isolate four fractions from the crude extract of M. pruriens seeds using hexane, EtOAc, BuOH, and water. All fractions were used to treat three cell lines: C6, Ea.hy926, and HCT-15. The water fraction significantly promoted nitric oxide (NO) production in C6 cells via neuronal nitric oxide synathase (nNOS) gene upregulation. M. pruriens fractions with high polarity promoted NO production in Ea.hy926 through the upregulation of endothelial NOS (eNOS) and inducible NOS (iNOS) genes. The BuOH and water fractions of M. pruriens extract were involved with cyclic GMP specific-phosphodiesterase (PDE5) and iNOS gene upregulation in HCT-15, which might result in maintenance of intracellular cGMP. According to the NIST library, 2,4-bis(1,1-dimethylethyl)-phenol, a phytochemical, may play a role in nNOS upregulation; this was only found in the water fraction. Protocatechuic acid, methyl ester, and γ-sitosterol were also found in the high-polar fractions of M. pruriens extract and might be involved with eNOS gene upregulation. The ability of M. pruriens extract to provoke NO production in neurons indicated the potential for using M. pruriens seeds as an alternative medicine for erectile dysfunction therapy. 2018-09-05T04:41:20Z 2018-09-05T04:41:20Z 2018-04-01 Journal 16851994 2-s2.0-85045298213 10.12982/CMUJNS.2018.0011 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85045298213&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/59203
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Multidisciplinary
spellingShingle Multidisciplinary
Natthachai Duangnin
Thanyaluck Phitak
Peraphan Pothacharoen
Prachya Kongtawelert
In vitro investigation of Mucuna pruriens seed extracts to treat erectile dysfunction
description © 2018, Chiang Mai University. M. pruriens seeds are a natural product with health benefits, including treating erectile dysfunction (ED). However, the active compounds and their mechanisms of action are unclear. In this study, we used polarity gradient extraction to isolate four fractions from the crude extract of M. pruriens seeds using hexane, EtOAc, BuOH, and water. All fractions were used to treat three cell lines: C6, Ea.hy926, and HCT-15. The water fraction significantly promoted nitric oxide (NO) production in C6 cells via neuronal nitric oxide synathase (nNOS) gene upregulation. M. pruriens fractions with high polarity promoted NO production in Ea.hy926 through the upregulation of endothelial NOS (eNOS) and inducible NOS (iNOS) genes. The BuOH and water fractions of M. pruriens extract were involved with cyclic GMP specific-phosphodiesterase (PDE5) and iNOS gene upregulation in HCT-15, which might result in maintenance of intracellular cGMP. According to the NIST library, 2,4-bis(1,1-dimethylethyl)-phenol, a phytochemical, may play a role in nNOS upregulation; this was only found in the water fraction. Protocatechuic acid, methyl ester, and γ-sitosterol were also found in the high-polar fractions of M. pruriens extract and might be involved with eNOS gene upregulation. The ability of M. pruriens extract to provoke NO production in neurons indicated the potential for using M. pruriens seeds as an alternative medicine for erectile dysfunction therapy.
format Journal
author Natthachai Duangnin
Thanyaluck Phitak
Peraphan Pothacharoen
Prachya Kongtawelert
author_facet Natthachai Duangnin
Thanyaluck Phitak
Peraphan Pothacharoen
Prachya Kongtawelert
author_sort Natthachai Duangnin
title In vitro investigation of Mucuna pruriens seed extracts to treat erectile dysfunction
title_short In vitro investigation of Mucuna pruriens seed extracts to treat erectile dysfunction
title_full In vitro investigation of Mucuna pruriens seed extracts to treat erectile dysfunction
title_fullStr In vitro investigation of Mucuna pruriens seed extracts to treat erectile dysfunction
title_full_unstemmed In vitro investigation of Mucuna pruriens seed extracts to treat erectile dysfunction
title_sort in vitro investigation of mucuna pruriens seed extracts to treat erectile dysfunction
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85045298213&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/59203
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