Flow injection analysis of total curcuminoids in turmeric and total antioxidant capacity using 2,2′-diphenyl-1-picrylhydrazyl assay

A simple flow injection analysis procedure is proposed for the determination of curcuminoids content in turmeric extracts. The method is based on the formation of a coloured complex between 4-aminoantipyrine and curcuminoids, in the presence of an oxidising reagent such as potassium hexacyanoferrate...

Full description

Saved in:
Bibliographic Details
Main Authors: Wisanu Thongchai, Boonsom Liawruangrath, Saisunee Liawruangrath
Format: Journal
Published: 2018
Subjects:
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=47949125479&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/59303
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Chiang Mai University
id th-cmuir.6653943832-59303
record_format dspace
spelling th-cmuir.6653943832-593032018-09-10T03:22:58Z Flow injection analysis of total curcuminoids in turmeric and total antioxidant capacity using 2,2′-diphenyl-1-picrylhydrazyl assay Wisanu Thongchai Boonsom Liawruangrath Saisunee Liawruangrath Agricultural and Biological Sciences Chemistry Medicine A simple flow injection analysis procedure is proposed for the determination of curcuminoids content in turmeric extracts. The method is based on the formation of a coloured complex between 4-aminoantipyrine and curcuminoids, in the presence of an oxidising reagent such as potassium hexacyanoferrate (III) in alkaline media. Conditions selected as a result of these trials were implemented in a flow injection analytical system in which the influence of injection volume, flow rate, reagent concentration and mixing coil length, was evaluated. Under the optimum conditions the total amount of curcuminoids could be determined within a concentration range of 5-50 μg mL-1which can be expressed by the regression equation y = 0.003x - 0.0053 (r2= 0.9997). The limits of detection and quantitation were found to be 0.6 μg mL-1and 1.8 μg mL-1, respectively. The reproducibility of analytical readings was indicative of standard deviations <2%. The sample was extracted and analysed by using the proposed method. The percentage recoveries were found to be 94.3-108.0. The proposed system was applied to the determination of curcuminoids content in turmeric. The total curcuminoid contents in turmeric extract were found to be 0.9-4.3% (w/w). The development method is simple, economic, rapid and especially suitable for quality control in pharmaceutical plants. © 2008 Elsevier Ltd. All rights reserved. 2018-09-10T03:13:37Z 2018-09-10T03:13:37Z 2009-01-15 Journal 03088146 2-s2.0-47949125479 10.1016/j.foodchem.2008.05.083 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=47949125479&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/59303
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Agricultural and Biological Sciences
Chemistry
Medicine
spellingShingle Agricultural and Biological Sciences
Chemistry
Medicine
Wisanu Thongchai
Boonsom Liawruangrath
Saisunee Liawruangrath
Flow injection analysis of total curcuminoids in turmeric and total antioxidant capacity using 2,2′-diphenyl-1-picrylhydrazyl assay
description A simple flow injection analysis procedure is proposed for the determination of curcuminoids content in turmeric extracts. The method is based on the formation of a coloured complex between 4-aminoantipyrine and curcuminoids, in the presence of an oxidising reagent such as potassium hexacyanoferrate (III) in alkaline media. Conditions selected as a result of these trials were implemented in a flow injection analytical system in which the influence of injection volume, flow rate, reagent concentration and mixing coil length, was evaluated. Under the optimum conditions the total amount of curcuminoids could be determined within a concentration range of 5-50 μg mL-1which can be expressed by the regression equation y = 0.003x - 0.0053 (r2= 0.9997). The limits of detection and quantitation were found to be 0.6 μg mL-1and 1.8 μg mL-1, respectively. The reproducibility of analytical readings was indicative of standard deviations <2%. The sample was extracted and analysed by using the proposed method. The percentage recoveries were found to be 94.3-108.0. The proposed system was applied to the determination of curcuminoids content in turmeric. The total curcuminoid contents in turmeric extract were found to be 0.9-4.3% (w/w). The development method is simple, economic, rapid and especially suitable for quality control in pharmaceutical plants. © 2008 Elsevier Ltd. All rights reserved.
format Journal
author Wisanu Thongchai
Boonsom Liawruangrath
Saisunee Liawruangrath
author_facet Wisanu Thongchai
Boonsom Liawruangrath
Saisunee Liawruangrath
author_sort Wisanu Thongchai
title Flow injection analysis of total curcuminoids in turmeric and total antioxidant capacity using 2,2′-diphenyl-1-picrylhydrazyl assay
title_short Flow injection analysis of total curcuminoids in turmeric and total antioxidant capacity using 2,2′-diphenyl-1-picrylhydrazyl assay
title_full Flow injection analysis of total curcuminoids in turmeric and total antioxidant capacity using 2,2′-diphenyl-1-picrylhydrazyl assay
title_fullStr Flow injection analysis of total curcuminoids in turmeric and total antioxidant capacity using 2,2′-diphenyl-1-picrylhydrazyl assay
title_full_unstemmed Flow injection analysis of total curcuminoids in turmeric and total antioxidant capacity using 2,2′-diphenyl-1-picrylhydrazyl assay
title_sort flow injection analysis of total curcuminoids in turmeric and total antioxidant capacity using 2,2′-diphenyl-1-picrylhydrazyl assay
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=47949125479&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/59303
_version_ 1681425226226728960