A modified hybridoma technique for production of monoclonal antibodies having desired isotypes

In the present study, we describe a modified hybridoma technique for production of monoclonal antibodies (mAbs) having a desired isotype. Mice were immunized with the antigen of interest. After having reached a high antibody titer, cells expressing IgM or IgG molecules were isolated from spleen cell...

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Main Authors: Napaporn Apiratmateekul, Ponrat Phunpae, Watchara Kasinrerk
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/59360
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-593602018-09-10T03:17:33Z A modified hybridoma technique for production of monoclonal antibodies having desired isotypes Napaporn Apiratmateekul Ponrat Phunpae Watchara Kasinrerk Biochemistry, Genetics and Molecular Biology Chemical Engineering Engineering In the present study, we describe a modified hybridoma technique for production of monoclonal antibodies (mAbs) having a desired isotype. Mice were immunized with the antigen of interest. After having reached a high antibody titer, cells expressing IgM or IgG molecules were isolated from spleen cells of the immunized mice using a Magnetic Cell Sorting System. The isolated cells were fused with myeloma cells using the conventional fusion protocol. With the isolated IgM+spleen cells, more than 75% (85 ± 7%; means ± SD) were IgM producing cells and a large number of IgM mAbs specific to the protein of interest were obtained. With the isolated IgG+spleen cells, 41 ± 40% of the generated hybridomas produced IgG antibody and no IgM producing hybridoma was generated. A large number of IgG mAbs specific to the protein of interest could be produced. The results indicate that the generated hybridomas produce corresponding antibody isotypes as expressed on the surface of their starting cells. The technique that we have developed will be very useful for production of desired mAbs having a specific isotype. © 2009 Springer Science+Business Media B.V. 2018-09-10T03:14:16Z 2018-09-10T03:14:16Z 2009-07-01 Journal 15730778 09209069 2-s2.0-71349083804 10.1007/s10616-009-9213-0 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=71349083804&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/59360
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
Chemical Engineering
Engineering
spellingShingle Biochemistry, Genetics and Molecular Biology
Chemical Engineering
Engineering
Napaporn Apiratmateekul
Ponrat Phunpae
Watchara Kasinrerk
A modified hybridoma technique for production of monoclonal antibodies having desired isotypes
description In the present study, we describe a modified hybridoma technique for production of monoclonal antibodies (mAbs) having a desired isotype. Mice were immunized with the antigen of interest. After having reached a high antibody titer, cells expressing IgM or IgG molecules were isolated from spleen cells of the immunized mice using a Magnetic Cell Sorting System. The isolated cells were fused with myeloma cells using the conventional fusion protocol. With the isolated IgM+spleen cells, more than 75% (85 ± 7%; means ± SD) were IgM producing cells and a large number of IgM mAbs specific to the protein of interest were obtained. With the isolated IgG+spleen cells, 41 ± 40% of the generated hybridomas produced IgG antibody and no IgM producing hybridoma was generated. A large number of IgG mAbs specific to the protein of interest could be produced. The results indicate that the generated hybridomas produce corresponding antibody isotypes as expressed on the surface of their starting cells. The technique that we have developed will be very useful for production of desired mAbs having a specific isotype. © 2009 Springer Science+Business Media B.V.
format Journal
author Napaporn Apiratmateekul
Ponrat Phunpae
Watchara Kasinrerk
author_facet Napaporn Apiratmateekul
Ponrat Phunpae
Watchara Kasinrerk
author_sort Napaporn Apiratmateekul
title A modified hybridoma technique for production of monoclonal antibodies having desired isotypes
title_short A modified hybridoma technique for production of monoclonal antibodies having desired isotypes
title_full A modified hybridoma technique for production of monoclonal antibodies having desired isotypes
title_fullStr A modified hybridoma technique for production of monoclonal antibodies having desired isotypes
title_full_unstemmed A modified hybridoma technique for production of monoclonal antibodies having desired isotypes
title_sort modified hybridoma technique for production of monoclonal antibodies having desired isotypes
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=71349083804&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/59360
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