Comparative study on methyl orange removal by growing cells and washed cell suspensions of Lactobacillus casei TISTR 1500

Lactobacillus casei TISTR 1500 possesses cytoplasmic azoreductase, and converts methyl orange to N,N-dimethyl-p-phenylenediamine and 4-aminobenzenesulfonic acid. In culture growth, the strain completely degraded methyl orange at 200 mg/l, even though the pH value was lower than 4. The decolorization...

Full description

Saved in:
Bibliographic Details
Main Authors: Phisit Seesuriyachan, Ampin Kuntiya, Ken Sasaki, Charin Techapun
Format: Journal
Published: 2018
Subjects:
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=67349276650&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/59365
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Chiang Mai University
id th-cmuir.6653943832-59365
record_format dspace
spelling th-cmuir.6653943832-593652018-09-10T03:18:36Z Comparative study on methyl orange removal by growing cells and washed cell suspensions of Lactobacillus casei TISTR 1500 Phisit Seesuriyachan Ampin Kuntiya Ken Sasaki Charin Techapun Biochemistry, Genetics and Molecular Biology Immunology and Microbiology Lactobacillus casei TISTR 1500 possesses cytoplasmic azoreductase, and converts methyl orange to N,N-dimethyl-p-phenylenediamine and 4-aminobenzenesulfonic acid. In culture growth, the strain completely degraded methyl orange at 200 mg/l, even though the pH value was lower than 4. The decolorization was inhibited in the growing culture with 800 mg of the dye/l after incubation for 12 h. The percentage of decolorization and specific decolorization rate with 400 and 800 mg/l were 66 and 15%, and 14.2, and 8.7 mg/gCell/h, respectively. Additionally, a growing culture is more tolerant to a high initial dye concentration than when using washed cell suspensions supplied with only sucrose. Moreover, incubation of a low cell density in 600 μM of Na+and 20 mM of sucrose increased the specific decolorization rate from 2.34 mg/gCell/h (without Na+) to 4.32 mg/gCell/h. However, Na+had no effect on the enhancement of azoreductase activity in the reaction mixture. © 2009 Springer Science+Business Media B.V. 2018-09-10T03:14:21Z 2018-09-10T03:14:21Z 2009-06-01 Journal 09593993 2-s2.0-67349276650 10.1007/s11274-009-9974-9 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=67349276650&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/59365
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
Immunology and Microbiology
spellingShingle Biochemistry, Genetics and Molecular Biology
Immunology and Microbiology
Phisit Seesuriyachan
Ampin Kuntiya
Ken Sasaki
Charin Techapun
Comparative study on methyl orange removal by growing cells and washed cell suspensions of Lactobacillus casei TISTR 1500
description Lactobacillus casei TISTR 1500 possesses cytoplasmic azoreductase, and converts methyl orange to N,N-dimethyl-p-phenylenediamine and 4-aminobenzenesulfonic acid. In culture growth, the strain completely degraded methyl orange at 200 mg/l, even though the pH value was lower than 4. The decolorization was inhibited in the growing culture with 800 mg of the dye/l after incubation for 12 h. The percentage of decolorization and specific decolorization rate with 400 and 800 mg/l were 66 and 15%, and 14.2, and 8.7 mg/gCell/h, respectively. Additionally, a growing culture is more tolerant to a high initial dye concentration than when using washed cell suspensions supplied with only sucrose. Moreover, incubation of a low cell density in 600 μM of Na+and 20 mM of sucrose increased the specific decolorization rate from 2.34 mg/gCell/h (without Na+) to 4.32 mg/gCell/h. However, Na+had no effect on the enhancement of azoreductase activity in the reaction mixture. © 2009 Springer Science+Business Media B.V.
format Journal
author Phisit Seesuriyachan
Ampin Kuntiya
Ken Sasaki
Charin Techapun
author_facet Phisit Seesuriyachan
Ampin Kuntiya
Ken Sasaki
Charin Techapun
author_sort Phisit Seesuriyachan
title Comparative study on methyl orange removal by growing cells and washed cell suspensions of Lactobacillus casei TISTR 1500
title_short Comparative study on methyl orange removal by growing cells and washed cell suspensions of Lactobacillus casei TISTR 1500
title_full Comparative study on methyl orange removal by growing cells and washed cell suspensions of Lactobacillus casei TISTR 1500
title_fullStr Comparative study on methyl orange removal by growing cells and washed cell suspensions of Lactobacillus casei TISTR 1500
title_full_unstemmed Comparative study on methyl orange removal by growing cells and washed cell suspensions of Lactobacillus casei TISTR 1500
title_sort comparative study on methyl orange removal by growing cells and washed cell suspensions of lactobacillus casei tistr 1500
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=67349276650&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/59365
_version_ 1681425237718073344