Closed-system solid surface vitrification versus slow programmable freezing of mouse 2-cell embryos
Purpose: To compare closed-system solid surface vitrification with slow freezing. Methods: Mouse 2-cell embryos (n=348) were divided into vitrification, slow freezing and non-frozen groups. For vitrification, embryos were exposed to 10% ethylene glycol (EG), 10% dimethylsulfoxide (DMSO) and 10% feta...
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| Main Authors: | , , , |
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| Format: | Journal |
| Published: |
2018
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| Subjects: | |
| Online Access: | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=70349600496&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/59371 |
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| Institution: | Chiang Mai University |
| Summary: | Purpose: To compare closed-system solid surface vitrification with slow freezing. Methods: Mouse 2-cell embryos (n=348) were divided into vitrification, slow freezing and non-frozen groups. For vitrification, embryos were exposed to 10% ethylene glycol (EG), 10% dimethylsulfoxide (DMSO) and 10% fetal bovine serum (FBS) in phosphate-buffered saline (PBS) for 10 min, then transferred into 17.5% EG, 17.5% DMSO, 0.25 M trehalose and 10% FBS in PBS. They were placed on hemi-straws and inserted into 0.5 ml straws inside a previously cooled aluminum cylinder. Slow freezing was done in straws by the conventional method. Results: Vitrified embryos had significantly higher survival, further cleavage and blastocyst formation rates than those in the slow freezing group (p<0.001) and were comparable to controls. Blastocysts in the vitrification and control groups had significantly more cells than those in the slow freezing group (p<0.05). Conclusions: Closed-system vitrification was more effective than conventional slow freezing. © 2009 Springer Science+Business Media, LLC. |
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