Sequential injection-capillary immunoassay system for determination of sialoglycoconjugates

An automatic immunoassay system for an assay of sialoglycoconjugates was developed based on the sequential injection technique. A cost effective plain glass capillary tube was used as a solid surface for immobilization of biomolecules via a simple physical adsorption which is adequate to tolerate th...

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Main Authors: Supaporn Kradtap Hartwell, Napaporn Wannaprom, Prachya Kongtawelert, Kate Grudpan
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/59457
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-594572018-09-10T03:15:29Z Sequential injection-capillary immunoassay system for determination of sialoglycoconjugates Supaporn Kradtap Hartwell Napaporn Wannaprom Prachya Kongtawelert Kate Grudpan Chemistry An automatic immunoassay system for an assay of sialoglycoconjugates was developed based on the sequential injection technique. A cost effective plain glass capillary tube was used as a solid surface for immobilization of biomolecules via a simple physical adsorption which is adequate to tolerate the force of solution flowing through the capillary during the multi-steps immunoassay process. Immunoassay could be performed with many improvements-rapidity per sample as compared to the conventional micro-plate format (40 min vs. 5-8 h); lower cost and simpler as compared to fused silica capillary with covalent immobilization; and without problem of back pressure as compared to flow injection-bead based immunoassay. Performance of the sequential injection-capillary immunoassay was demonstrated by assay of sialoglycoconjugates level in human serum to differentiate cancer patients from healthy people. © 2009. 2018-09-10T03:15:29Z 2018-09-10T03:15:29Z 2009-10-15 Journal 00399140 2-s2.0-67650991817 10.1016/j.talanta.2009.04.020 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=67650991817&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/59457
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Chemistry
spellingShingle Chemistry
Supaporn Kradtap Hartwell
Napaporn Wannaprom
Prachya Kongtawelert
Kate Grudpan
Sequential injection-capillary immunoassay system for determination of sialoglycoconjugates
description An automatic immunoassay system for an assay of sialoglycoconjugates was developed based on the sequential injection technique. A cost effective plain glass capillary tube was used as a solid surface for immobilization of biomolecules via a simple physical adsorption which is adequate to tolerate the force of solution flowing through the capillary during the multi-steps immunoassay process. Immunoassay could be performed with many improvements-rapidity per sample as compared to the conventional micro-plate format (40 min vs. 5-8 h); lower cost and simpler as compared to fused silica capillary with covalent immobilization; and without problem of back pressure as compared to flow injection-bead based immunoassay. Performance of the sequential injection-capillary immunoassay was demonstrated by assay of sialoglycoconjugates level in human serum to differentiate cancer patients from healthy people. © 2009.
format Journal
author Supaporn Kradtap Hartwell
Napaporn Wannaprom
Prachya Kongtawelert
Kate Grudpan
author_facet Supaporn Kradtap Hartwell
Napaporn Wannaprom
Prachya Kongtawelert
Kate Grudpan
author_sort Supaporn Kradtap Hartwell
title Sequential injection-capillary immunoassay system for determination of sialoglycoconjugates
title_short Sequential injection-capillary immunoassay system for determination of sialoglycoconjugates
title_full Sequential injection-capillary immunoassay system for determination of sialoglycoconjugates
title_fullStr Sequential injection-capillary immunoassay system for determination of sialoglycoconjugates
title_full_unstemmed Sequential injection-capillary immunoassay system for determination of sialoglycoconjugates
title_sort sequential injection-capillary immunoassay system for determination of sialoglycoconjugates
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=67650991817&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/59457
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