Development of genotype-specific primers for differentiation of genotypes A and B of Aichi viruses

A nested polymerase chain reaction method using genotype-specific primers based on the capsid gene was developed to differentiate between genotypes A and B of Aichi viruses. Results of the study showed that the PCR using newly designed genotype-specific primers could generate appropriate PCR product...

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Main Authors: Ngan Thi Kim Pham, Quang Duy Trinh, Tuan Anh Nguyen, Shuvra Kanti Dey, Tung Gia Phan, Le Phuc Hoang, Pattara Khamrin, Niwat Maneekarn, Shoko Okitsu, Masashi Mizuguchi, Hiroshi Ushijima
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/59641
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-596412018-09-10T03:18:45Z Development of genotype-specific primers for differentiation of genotypes A and B of Aichi viruses Ngan Thi Kim Pham Quang Duy Trinh Tuan Anh Nguyen Shuvra Kanti Dey Tung Gia Phan Le Phuc Hoang Pattara Khamrin Niwat Maneekarn Shoko Okitsu Masashi Mizuguchi Hiroshi Ushijima Immunology and Microbiology A nested polymerase chain reaction method using genotype-specific primers based on the capsid gene was developed to differentiate between genotypes A and B of Aichi viruses. Results of the study showed that the PCR using newly designed genotype-specific primers could generate appropriate PCR products from all 17 samples tested, the newly developed primers could differentiate genotype A from genotype B, and all matched those obtained by nucleotide sequencing of the capsid regions. The nested PCR method using genotype-specific primers is useful and can be used for genotyping of Aichi viruses isolated from epidemiological studies. © 2008 Elsevier B.V. All rights reserved. 2018-09-10T03:18:45Z 2018-09-10T03:18:45Z 2009-03-01 Journal 01660934 2-s2.0-59749093977 10.1016/j.jviromet.2008.11.005 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=59749093977&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/59641
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Immunology and Microbiology
spellingShingle Immunology and Microbiology
Ngan Thi Kim Pham
Quang Duy Trinh
Tuan Anh Nguyen
Shuvra Kanti Dey
Tung Gia Phan
Le Phuc Hoang
Pattara Khamrin
Niwat Maneekarn
Shoko Okitsu
Masashi Mizuguchi
Hiroshi Ushijima
Development of genotype-specific primers for differentiation of genotypes A and B of Aichi viruses
description A nested polymerase chain reaction method using genotype-specific primers based on the capsid gene was developed to differentiate between genotypes A and B of Aichi viruses. Results of the study showed that the PCR using newly designed genotype-specific primers could generate appropriate PCR products from all 17 samples tested, the newly developed primers could differentiate genotype A from genotype B, and all matched those obtained by nucleotide sequencing of the capsid regions. The nested PCR method using genotype-specific primers is useful and can be used for genotyping of Aichi viruses isolated from epidemiological studies. © 2008 Elsevier B.V. All rights reserved.
format Journal
author Ngan Thi Kim Pham
Quang Duy Trinh
Tuan Anh Nguyen
Shuvra Kanti Dey
Tung Gia Phan
Le Phuc Hoang
Pattara Khamrin
Niwat Maneekarn
Shoko Okitsu
Masashi Mizuguchi
Hiroshi Ushijima
author_facet Ngan Thi Kim Pham
Quang Duy Trinh
Tuan Anh Nguyen
Shuvra Kanti Dey
Tung Gia Phan
Le Phuc Hoang
Pattara Khamrin
Niwat Maneekarn
Shoko Okitsu
Masashi Mizuguchi
Hiroshi Ushijima
author_sort Ngan Thi Kim Pham
title Development of genotype-specific primers for differentiation of genotypes A and B of Aichi viruses
title_short Development of genotype-specific primers for differentiation of genotypes A and B of Aichi viruses
title_full Development of genotype-specific primers for differentiation of genotypes A and B of Aichi viruses
title_fullStr Development of genotype-specific primers for differentiation of genotypes A and B of Aichi viruses
title_full_unstemmed Development of genotype-specific primers for differentiation of genotypes A and B of Aichi viruses
title_sort development of genotype-specific primers for differentiation of genotypes a and b of aichi viruses
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=59749093977&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/59641
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