Analysis of the VP6 gene of human and porcine group A rotavirus strains with unusual subgroup specificities
Full-length VP6 amino acid sequences of human and porcine rotaviruses with subgroup (SG) (I + II) and SG non-(I + II) were analyzed in comparison with those of SG I and SG II. In human rotaviruses, the strains in the same SG shared a very high degree of amino acid identity, ranging from 97.4% to 99....
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th-cmuir.6653943832-596472018-09-10T03:23:17Z Analysis of the VP6 gene of human and porcine group A rotavirus strains with unusual subgroup specificities Aksara Thongprachum Pattara Khamrin Prayuth Saekhow Chansom Pantip Supatra Peerakome Hiroshi Ushijima Niwat Maneekarn Immunology and Microbiology Medicine Full-length VP6 amino acid sequences of human and porcine rotaviruses with subgroup (SG) (I + II) and SG non-(I + II) were analyzed in comparison with those of SG I and SG II. In human rotaviruses, the strains in the same SG shared a very high degree of amino acid identity, ranging from 97.4% to 99.4% for SG I, 95.9% to 100% for SG II, and 99.4% to 100% for SG non-(I + II), while viruses in different SGs shared somewhat lower sequence identity at 90.4-93.1%. Conserved amino acids that distinguished the strains of SG I from SG II were observed at 21 positions. The viruses with SG non-(I + II) shared sequence identity with SG II as high as 97.2-99.7%, suggesting that they belonged to genogroup II. Similarly, porcine rotaviruses in the same SG shared 96.4-99.7% for SG I, 98.2-100% for SG II, 97.4-100% for SG (I + II), and 96.2-99.7% for SG non-(I + II), while strains in different SGs shared sequence identity ranging from 91.9% to 94.4%. Interestingly, the strains with SG (I + II) and SG non-(I + II) shared a high degree of sequence identity with SG I, at 96.4-100% and 94.7-99.7% respectively, suggesting that they are related to porcine SG I strains. The conserved amino acids which distinguished SG I from SG II were observed at 13 positions. The strains with SG I, SG (I + II), and SG non-(I + II) showed identical amino acid residues at these positions. Phylogenetic analysis strongly supported the findings of the sequence analysis. © 2008 Wiley-Liss, Inc. 2018-09-10T03:18:51Z 2018-09-10T03:18:51Z 2009-01-01 Journal 10969071 01466615 2-s2.0-58149265791 10.1002/jmv.21345 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=58149265791&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/59647 |
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Immunology and Microbiology Medicine Aksara Thongprachum Pattara Khamrin Prayuth Saekhow Chansom Pantip Supatra Peerakome Hiroshi Ushijima Niwat Maneekarn Analysis of the VP6 gene of human and porcine group A rotavirus strains with unusual subgroup specificities |
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Full-length VP6 amino acid sequences of human and porcine rotaviruses with subgroup (SG) (I + II) and SG non-(I + II) were analyzed in comparison with those of SG I and SG II. In human rotaviruses, the strains in the same SG shared a very high degree of amino acid identity, ranging from 97.4% to 99.4% for SG I, 95.9% to 100% for SG II, and 99.4% to 100% for SG non-(I + II), while viruses in different SGs shared somewhat lower sequence identity at 90.4-93.1%. Conserved amino acids that distinguished the strains of SG I from SG II were observed at 21 positions. The viruses with SG non-(I + II) shared sequence identity with SG II as high as 97.2-99.7%, suggesting that they belonged to genogroup II. Similarly, porcine rotaviruses in the same SG shared 96.4-99.7% for SG I, 98.2-100% for SG II, 97.4-100% for SG (I + II), and 96.2-99.7% for SG non-(I + II), while strains in different SGs shared sequence identity ranging from 91.9% to 94.4%. Interestingly, the strains with SG (I + II) and SG non-(I + II) shared a high degree of sequence identity with SG I, at 96.4-100% and 94.7-99.7% respectively, suggesting that they are related to porcine SG I strains. The conserved amino acids which distinguished SG I from SG II were observed at 13 positions. The strains with SG I, SG (I + II), and SG non-(I + II) showed identical amino acid residues at these positions. Phylogenetic analysis strongly supported the findings of the sequence analysis. © 2008 Wiley-Liss, Inc. |
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Journal |
author |
Aksara Thongprachum Pattara Khamrin Prayuth Saekhow Chansom Pantip Supatra Peerakome Hiroshi Ushijima Niwat Maneekarn |
author_facet |
Aksara Thongprachum Pattara Khamrin Prayuth Saekhow Chansom Pantip Supatra Peerakome Hiroshi Ushijima Niwat Maneekarn |
author_sort |
Aksara Thongprachum |
title |
Analysis of the VP6 gene of human and porcine group A rotavirus strains with unusual subgroup specificities |
title_short |
Analysis of the VP6 gene of human and porcine group A rotavirus strains with unusual subgroup specificities |
title_full |
Analysis of the VP6 gene of human and porcine group A rotavirus strains with unusual subgroup specificities |
title_fullStr |
Analysis of the VP6 gene of human and porcine group A rotavirus strains with unusual subgroup specificities |
title_full_unstemmed |
Analysis of the VP6 gene of human and porcine group A rotavirus strains with unusual subgroup specificities |
title_sort |
analysis of the vp6 gene of human and porcine group a rotavirus strains with unusual subgroup specificities |
publishDate |
2018 |
url |
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=58149265791&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/59647 |
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1681425290046210048 |