Application of nested PCR to detect Penicillium marneffei in serum samples
We previously reported a nested PCR assay for specific identification of 18S ribosomal DNA of Penicillium marneffei. In this study, the assay was used to detect the DNA of P. marneffei in serum samples. Sensitivity of the test was 4 pg/l and 0.4 fg/l when the cycle numbers used for nested reactions...
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th-cmuir.6653943832-597552018-09-10T03:25:52Z Application of nested PCR to detect Penicillium marneffei in serum samples Monsicha Pongpom Thira Sirisanthana Nongnuch Vanittanakom Medicine Veterinary We previously reported a nested PCR assay for specific identification of 18S ribosomal DNA of Penicillium marneffei. In this study, the assay was used to detect the DNA of P. marneffei in serum samples. Sensitivity of the test was 4 pg/l and 0.4 fg/l when the cycle numbers used for nested reactions were 15 and 30, respectively. Twenty four out of 35 sera (68.6%) collected from patients with culture confirmed penicilliosis marneffei were positive, while normal healthy and non-P. marneffei infected HIV-positive sera were negative. The results suggested that the assay could be applied for the diagnosis of infections due to P. marneffei. © 2009 ISHAM. 2018-09-10T03:21:06Z 2018-09-10T03:21:06Z 2009-12-03 Journal 14602709 13693786 2-s2.0-70749124150 10.1080/13693780802484875 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=70749124150&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/59755 |
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Medicine Veterinary Monsicha Pongpom Thira Sirisanthana Nongnuch Vanittanakom Application of nested PCR to detect Penicillium marneffei in serum samples |
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We previously reported a nested PCR assay for specific identification of 18S ribosomal DNA of Penicillium marneffei. In this study, the assay was used to detect the DNA of P. marneffei in serum samples. Sensitivity of the test was 4 pg/l and 0.4 fg/l when the cycle numbers used for nested reactions were 15 and 30, respectively. Twenty four out of 35 sera (68.6%) collected from patients with culture confirmed penicilliosis marneffei were positive, while normal healthy and non-P. marneffei infected HIV-positive sera were negative. The results suggested that the assay could be applied for the diagnosis of infections due to P. marneffei. © 2009 ISHAM. |
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Monsicha Pongpom Thira Sirisanthana Nongnuch Vanittanakom |
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Monsicha Pongpom Thira Sirisanthana Nongnuch Vanittanakom |
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Monsicha Pongpom |
title |
Application of nested PCR to detect Penicillium marneffei in serum samples |
title_short |
Application of nested PCR to detect Penicillium marneffei in serum samples |
title_full |
Application of nested PCR to detect Penicillium marneffei in serum samples |
title_fullStr |
Application of nested PCR to detect Penicillium marneffei in serum samples |
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Application of nested PCR to detect Penicillium marneffei in serum samples |
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application of nested pcr to detect penicillium marneffei in serum samples |
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2018 |
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https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=70749124150&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/59755 |
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