Application of nested PCR to detect Penicillium marneffei in serum samples

We previously reported a nested PCR assay for specific identification of 18S ribosomal DNA of Penicillium marneffei. In this study, the assay was used to detect the DNA of P. marneffei in serum samples. Sensitivity of the test was 4 pg/l and 0.4 fg/l when the cycle numbers used for nested reactions...

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Main Authors: Monsicha Pongpom, Thira Sirisanthana, Nongnuch Vanittanakom
Format: Journal
Published: 2018
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Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=70749124150&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/59755
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-597552018-09-10T03:25:52Z Application of nested PCR to detect Penicillium marneffei in serum samples Monsicha Pongpom Thira Sirisanthana Nongnuch Vanittanakom Medicine Veterinary We previously reported a nested PCR assay for specific identification of 18S ribosomal DNA of Penicillium marneffei. In this study, the assay was used to detect the DNA of P. marneffei in serum samples. Sensitivity of the test was 4 pg/l and 0.4 fg/l when the cycle numbers used for nested reactions were 15 and 30, respectively. Twenty four out of 35 sera (68.6%) collected from patients with culture confirmed penicilliosis marneffei were positive, while normal healthy and non-P. marneffei infected HIV-positive sera were negative. The results suggested that the assay could be applied for the diagnosis of infections due to P. marneffei. © 2009 ISHAM. 2018-09-10T03:21:06Z 2018-09-10T03:21:06Z 2009-12-03 Journal 14602709 13693786 2-s2.0-70749124150 10.1080/13693780802484875 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=70749124150&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/59755
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Medicine
Veterinary
spellingShingle Medicine
Veterinary
Monsicha Pongpom
Thira Sirisanthana
Nongnuch Vanittanakom
Application of nested PCR to detect Penicillium marneffei in serum samples
description We previously reported a nested PCR assay for specific identification of 18S ribosomal DNA of Penicillium marneffei. In this study, the assay was used to detect the DNA of P. marneffei in serum samples. Sensitivity of the test was 4 pg/l and 0.4 fg/l when the cycle numbers used for nested reactions were 15 and 30, respectively. Twenty four out of 35 sera (68.6%) collected from patients with culture confirmed penicilliosis marneffei were positive, while normal healthy and non-P. marneffei infected HIV-positive sera were negative. The results suggested that the assay could be applied for the diagnosis of infections due to P. marneffei. © 2009 ISHAM.
format Journal
author Monsicha Pongpom
Thira Sirisanthana
Nongnuch Vanittanakom
author_facet Monsicha Pongpom
Thira Sirisanthana
Nongnuch Vanittanakom
author_sort Monsicha Pongpom
title Application of nested PCR to detect Penicillium marneffei in serum samples
title_short Application of nested PCR to detect Penicillium marneffei in serum samples
title_full Application of nested PCR to detect Penicillium marneffei in serum samples
title_fullStr Application of nested PCR to detect Penicillium marneffei in serum samples
title_full_unstemmed Application of nested PCR to detect Penicillium marneffei in serum samples
title_sort application of nested pcr to detect penicillium marneffei in serum samples
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=70749124150&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/59755
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