Application of nested PCR to detect Penicillium marneffei in serum samples

We previously reported a nested PCR assay for specific identification of 18S ribosomal DNA of Penicillium marneffei. In this study, the assay was used to detect the DNA of P. marneffei in serum samples. Sensitivity of the test was 4 pg/l and 0.4 fg/l when the cycle numbers used for nested reactions...

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Main Authors:	Monsicha Pongpom, Thira Sirisanthana, Nongnuch Vanittanakom
Format:	Journal
Published:	2018
Subjects:	Medicine Veterinary
Online Access:	https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=70749124150&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/59755
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Institution:	Chiang Mai University

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spelling	th-cmuir.6653943832-597552018-09-10T03:25:52Z Application of nested PCR to detect Penicillium marneffei in serum samples Monsicha Pongpom Thira Sirisanthana Nongnuch Vanittanakom Medicine Veterinary We previously reported a nested PCR assay for specific identification of 18S ribosomal DNA of Penicillium marneffei. In this study, the assay was used to detect the DNA of P. marneffei in serum samples. Sensitivity of the test was 4 pg/l and 0.4 fg/l when the cycle numbers used for nested reactions were 15 and 30, respectively. Twenty four out of 35 sera (68.6%) collected from patients with culture confirmed penicilliosis marneffei were positive, while normal healthy and non-P. marneffei infected HIV-positive sera were negative. The results suggested that the assay could be applied for the diagnosis of infections due to P. marneffei. © 2009 ISHAM. 2018-09-10T03:21:06Z 2018-09-10T03:21:06Z 2009-12-03 Journal 14602709 13693786 2-s2.0-70749124150 10.1080/13693780802484875 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=70749124150&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/59755
institution	Chiang Mai University
building	Chiang Mai University Library
country	Thailand
collection	CMU Intellectual Repository
topic	Medicine Veterinary
spellingShingle	Medicine Veterinary Monsicha Pongpom Thira Sirisanthana Nongnuch Vanittanakom Application of nested PCR to detect Penicillium marneffei in serum samples
description	We previously reported a nested PCR assay for specific identification of 18S ribosomal DNA of Penicillium marneffei. In this study, the assay was used to detect the DNA of P. marneffei in serum samples. Sensitivity of the test was 4 pg/l and 0.4 fg/l when the cycle numbers used for nested reactions were 15 and 30, respectively. Twenty four out of 35 sera (68.6%) collected from patients with culture confirmed penicilliosis marneffei were positive, while normal healthy and non-P. marneffei infected HIV-positive sera were negative. The results suggested that the assay could be applied for the diagnosis of infections due to P. marneffei. © 2009 ISHAM.
format	Journal
author	Monsicha Pongpom Thira Sirisanthana Nongnuch Vanittanakom
author_facet	Monsicha Pongpom Thira Sirisanthana Nongnuch Vanittanakom
author_sort	Monsicha Pongpom
title	Application of nested PCR to detect Penicillium marneffei in serum samples
title_short	Application of nested PCR to detect Penicillium marneffei in serum samples
title_full	Application of nested PCR to detect Penicillium marneffei in serum samples
title_fullStr	Application of nested PCR to detect Penicillium marneffei in serum samples
title_full_unstemmed	Application of nested PCR to detect Penicillium marneffei in serum samples
title_sort	application of nested pcr to detect penicillium marneffei in serum samples
publishDate	2018
url	https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=70749124150&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/59755
_version_	1681425310042554368

Application of nested PCR to detect Penicillium marneffei in serum samples

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