Isolation and expression of heat shock protein 30 gene from Penicillium marneffei

Penicillium marneffei is a dimorphic fungus that can cause disseminated mycosis, especially in AIDS patients. The role of heat shock proteins and stress response-related proteins in P. marneffei remains unknown. In this study, we isolated a cDNA encoding for heat shock protein 30 (Hsp30) of P. marne...

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Main Authors: Nongnuch Vanittanakom, Monsicha Pongpom, Jutarat Praparattanapan, Chester R. Cooper, Thira Sirisanthana
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/59756
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-597562018-09-10T03:25:53Z Isolation and expression of heat shock protein 30 gene from Penicillium marneffei Nongnuch Vanittanakom Monsicha Pongpom Jutarat Praparattanapan Chester R. Cooper Thira Sirisanthana Medicine Veterinary Penicillium marneffei is a dimorphic fungus that can cause disseminated mycosis, especially in AIDS patients. The role of heat shock proteins and stress response-related proteins in P. marneffei remains unknown. In this study, we isolated a cDNA encoding for heat shock protein 30 (Hsp30) of P. marneffei using an antibody screening method. The DNA sequence and deduced amino acid sequence analysis showed high homology to other fungal hsp30 genes. Expression of P. marneffei hsp30 in response to temperature increase was determined by Northern blot analysis. A high level of hsp30 transcript was detected in yeast cells grown at 37°C, whereas a very low or undetectable transcript level was observed in mycelial cells at 25°C. A recombinant Hsp30 protein was produced and tested preliminarily for its immunoreactivity with sera from P. marneffei-infected AIDS patients using Western blot analysis. The positive immunoblot result, with some serum samples, confirmed the antigenic property of the Hsp30. Collectively, the high response of hsp30 to temperature increase could indicate it may play a role in heat stress response and cell adaptation. This is the first report showing that this small heat shock protein could elicit the human immune response. © 2009 ISHAM. 2018-09-10T03:21:07Z 2018-09-10T03:21:07Z 2009-12-03 Journal 14602709 13693786 2-s2.0-70749160462 10.1080/13693780802566358 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=70749160462&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/59756
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Medicine
Veterinary
spellingShingle Medicine
Veterinary
Nongnuch Vanittanakom
Monsicha Pongpom
Jutarat Praparattanapan
Chester R. Cooper
Thira Sirisanthana
Isolation and expression of heat shock protein 30 gene from Penicillium marneffei
description Penicillium marneffei is a dimorphic fungus that can cause disseminated mycosis, especially in AIDS patients. The role of heat shock proteins and stress response-related proteins in P. marneffei remains unknown. In this study, we isolated a cDNA encoding for heat shock protein 30 (Hsp30) of P. marneffei using an antibody screening method. The DNA sequence and deduced amino acid sequence analysis showed high homology to other fungal hsp30 genes. Expression of P. marneffei hsp30 in response to temperature increase was determined by Northern blot analysis. A high level of hsp30 transcript was detected in yeast cells grown at 37°C, whereas a very low or undetectable transcript level was observed in mycelial cells at 25°C. A recombinant Hsp30 protein was produced and tested preliminarily for its immunoreactivity with sera from P. marneffei-infected AIDS patients using Western blot analysis. The positive immunoblot result, with some serum samples, confirmed the antigenic property of the Hsp30. Collectively, the high response of hsp30 to temperature increase could indicate it may play a role in heat stress response and cell adaptation. This is the first report showing that this small heat shock protein could elicit the human immune response. © 2009 ISHAM.
format Journal
author Nongnuch Vanittanakom
Monsicha Pongpom
Jutarat Praparattanapan
Chester R. Cooper
Thira Sirisanthana
author_facet Nongnuch Vanittanakom
Monsicha Pongpom
Jutarat Praparattanapan
Chester R. Cooper
Thira Sirisanthana
author_sort Nongnuch Vanittanakom
title Isolation and expression of heat shock protein 30 gene from Penicillium marneffei
title_short Isolation and expression of heat shock protein 30 gene from Penicillium marneffei
title_full Isolation and expression of heat shock protein 30 gene from Penicillium marneffei
title_fullStr Isolation and expression of heat shock protein 30 gene from Penicillium marneffei
title_full_unstemmed Isolation and expression of heat shock protein 30 gene from Penicillium marneffei
title_sort isolation and expression of heat shock protein 30 gene from penicillium marneffei
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=70749160462&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/59756
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