Development of PCR-based diagnosis of minute intestinal fluke, Haplorchis taichui

Haplorchis taichui specific primers were designed using a high annealing temperature random amplified polymorphic DNA (HAT-RAPD) PCR method and 18 arbitrary primers (Operon Technologies) to generate polymorphic DNA profiles for 13 different parasites. The H. taichui specific fragment was screened. A...

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Main Authors: Pheravut Wongsawad, Chalobol Wongsawad
Format: Journal
Published: 2018
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Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=70349789724&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/59801
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-598012018-09-10T03:21:41Z Development of PCR-based diagnosis of minute intestinal fluke, Haplorchis taichui Pheravut Wongsawad Chalobol Wongsawad Medicine Haplorchis taichui specific primers were designed using a high annealing temperature random amplified polymorphic DNA (HAT-RAPD) PCR method and 18 arbitrary primers (Operon Technologies) to generate polymorphic DNA profiles for 13 different parasites. The H. taichui specific fragment was screened. A 256 bp HAT-RAPD marker generated from OPP-11 primer specific for H. taichui was cloned and sequenced. From the sequence data, specific primers were designed that generated a 256 bp amplicon. The minimum DNA template needed for PCR detection was 10 fg. The successful development of the H. taichui specific DNA-based detection will be beneficial in management and epidemiological control programs. 2018-09-10T03:21:41Z 2018-09-10T03:21:41Z 2009-09-01 Journal 01251562 2-s2.0-70349789724 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=70349789724&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/59801
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Medicine
spellingShingle Medicine
Pheravut Wongsawad
Chalobol Wongsawad
Development of PCR-based diagnosis of minute intestinal fluke, Haplorchis taichui
description Haplorchis taichui specific primers were designed using a high annealing temperature random amplified polymorphic DNA (HAT-RAPD) PCR method and 18 arbitrary primers (Operon Technologies) to generate polymorphic DNA profiles for 13 different parasites. The H. taichui specific fragment was screened. A 256 bp HAT-RAPD marker generated from OPP-11 primer specific for H. taichui was cloned and sequenced. From the sequence data, specific primers were designed that generated a 256 bp amplicon. The minimum DNA template needed for PCR detection was 10 fg. The successful development of the H. taichui specific DNA-based detection will be beneficial in management and epidemiological control programs.
format Journal
author Pheravut Wongsawad
Chalobol Wongsawad
author_facet Pheravut Wongsawad
Chalobol Wongsawad
author_sort Pheravut Wongsawad
title Development of PCR-based diagnosis of minute intestinal fluke, Haplorchis taichui
title_short Development of PCR-based diagnosis of minute intestinal fluke, Haplorchis taichui
title_full Development of PCR-based diagnosis of minute intestinal fluke, Haplorchis taichui
title_fullStr Development of PCR-based diagnosis of minute intestinal fluke, Haplorchis taichui
title_full_unstemmed Development of PCR-based diagnosis of minute intestinal fluke, Haplorchis taichui
title_sort development of pcr-based diagnosis of minute intestinal fluke, haplorchis taichui
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=70349789724&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/59801
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