Development of PCR-based diagnosis of minute intestinal fluke, Haplorchis taichui

Haplorchis taichui specific primers were designed using a high annealing temperature random amplified polymorphic DNA (HAT-RAPD) PCR method and 18 arbitrary primers (Operon Technologies) to generate polymorphic DNA profiles for 13 different parasites. The H. taichui specific fragment was screened. A...

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Main Authors:	Pheravut Wongsawad, Chalobol Wongsawad
Format:	Journal
Published:	2018
Subjects:	Medicine
Online Access:	https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=70349789724&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/59801
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Institution:	Chiang Mai University

id	th-cmuir.6653943832-59801
record_format	dspace
spelling	th-cmuir.6653943832-598012018-09-10T03:21:41Z Development of PCR-based diagnosis of minute intestinal fluke, Haplorchis taichui Pheravut Wongsawad Chalobol Wongsawad Medicine Haplorchis taichui specific primers were designed using a high annealing temperature random amplified polymorphic DNA (HAT-RAPD) PCR method and 18 arbitrary primers (Operon Technologies) to generate polymorphic DNA profiles for 13 different parasites. The H. taichui specific fragment was screened. A 256 bp HAT-RAPD marker generated from OPP-11 primer specific for H. taichui was cloned and sequenced. From the sequence data, specific primers were designed that generated a 256 bp amplicon. The minimum DNA template needed for PCR detection was 10 fg. The successful development of the H. taichui specific DNA-based detection will be beneficial in management and epidemiological control programs. 2018-09-10T03:21:41Z 2018-09-10T03:21:41Z 2009-09-01 Journal 01251562 2-s2.0-70349789724 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=70349789724&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/59801
institution	Chiang Mai University
building	Chiang Mai University Library
country	Thailand
collection	CMU Intellectual Repository
topic	Medicine
spellingShingle	Medicine Pheravut Wongsawad Chalobol Wongsawad Development of PCR-based diagnosis of minute intestinal fluke, Haplorchis taichui
description	Haplorchis taichui specific primers were designed using a high annealing temperature random amplified polymorphic DNA (HAT-RAPD) PCR method and 18 arbitrary primers (Operon Technologies) to generate polymorphic DNA profiles for 13 different parasites. The H. taichui specific fragment was screened. A 256 bp HAT-RAPD marker generated from OPP-11 primer specific for H. taichui was cloned and sequenced. From the sequence data, specific primers were designed that generated a 256 bp amplicon. The minimum DNA template needed for PCR detection was 10 fg. The successful development of the H. taichui specific DNA-based detection will be beneficial in management and epidemiological control programs.
format	Journal
author	Pheravut Wongsawad Chalobol Wongsawad
author_facet	Pheravut Wongsawad Chalobol Wongsawad
author_sort	Pheravut Wongsawad
title	Development of PCR-based diagnosis of minute intestinal fluke, Haplorchis taichui
title_short	Development of PCR-based diagnosis of minute intestinal fluke, Haplorchis taichui
title_full	Development of PCR-based diagnosis of minute intestinal fluke, Haplorchis taichui
title_fullStr	Development of PCR-based diagnosis of minute intestinal fluke, Haplorchis taichui
title_full_unstemmed	Development of PCR-based diagnosis of minute intestinal fluke, Haplorchis taichui
title_sort	development of pcr-based diagnosis of minute intestinal fluke, haplorchis taichui
publishDate	2018
url	https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=70349789724&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/59801
_version_	1681425318537068544

Development of PCR-based diagnosis of minute intestinal fluke, Haplorchis taichui

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