Copro-diagnosis of Haplorchis taichui infection using sedimentation and PCR-based methods

Copro-diagnosis of Haplorchis taichui infection using sedimentation and PCR-based methods

Fifty-one human fecal specimens were collected from villagers inhabiting along Mae Kuang River, Ban Sob Tha, Pa Sang District, Lamphun Province, Thailand. By the formalin-ether sedimentation technique (FEST) under a light microscope, eggs of 3 helminth species, Haplorchis taichui, Ascaris lumbricoid...

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Main Authors: Chalobol Wongsawad, Pheravut Wongsawad, Suksan Chuboon, Somboon Anuntalabhochai
Format: Journal
Published: 2018
Subjects:
Medicine
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=70349829696&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/59803
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Institution: Chiang Mai University
id th-cmuir.6653943832-59803
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spelling th-cmuir.6653943832-598032018-09-10T03:21:42Z Copro-diagnosis of Haplorchis taichui infection using sedimentation and PCR-based methods Chalobol Wongsawad Pheravut Wongsawad Suksan Chuboon Somboon Anuntalabhochai Medicine Fifty-one human fecal specimens were collected from villagers inhabiting along Mae Kuang River, Ban Sob Tha, Pa Sang District, Lamphun Province, Thailand. By the formalin-ether sedimentation technique (FEST) under a light microscope, eggs of 3 helminth species, Haplorchis taichui, Ascaris lumbricoides and unidentified hookworm species were detected with prevalences of 22,14 and 4%, respectively. PCR amplification with H. taichui specific primers showed that H. taichui specific amplicon 260 bp was generated in all FEST-positive specimens, and also in some FEST negative specimens. This H. taichui specific PCR method can be used to detect this parasite in all developmental stages and in both definitive and intermediate hosts, which should be useful in prevention and control programs. 2018-09-10T03:21:42Z 2018-09-10T03:21:42Z 2009-09-01 Journal 01251562 2-s2.0-70349829696 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=70349829696&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/59803
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Medicine
spellingShingle Medicine
Chalobol Wongsawad
Pheravut Wongsawad
Suksan Chuboon
Somboon Anuntalabhochai
Copro-diagnosis of Haplorchis taichui infection using sedimentation and PCR-based methods
description Fifty-one human fecal specimens were collected from villagers inhabiting along Mae Kuang River, Ban Sob Tha, Pa Sang District, Lamphun Province, Thailand. By the formalin-ether sedimentation technique (FEST) under a light microscope, eggs of 3 helminth species, Haplorchis taichui, Ascaris lumbricoides and unidentified hookworm species were detected with prevalences of 22,14 and 4%, respectively. PCR amplification with H. taichui specific primers showed that H. taichui specific amplicon 260 bp was generated in all FEST-positive specimens, and also in some FEST negative specimens. This H. taichui specific PCR method can be used to detect this parasite in all developmental stages and in both definitive and intermediate hosts, which should be useful in prevention and control programs.
format Journal
author Chalobol Wongsawad
Pheravut Wongsawad
Suksan Chuboon
Somboon Anuntalabhochai
author_facet Chalobol Wongsawad
Pheravut Wongsawad
Suksan Chuboon
Somboon Anuntalabhochai
author_sort Chalobol Wongsawad
title Copro-diagnosis of Haplorchis taichui infection using sedimentation and PCR-based methods
title_short Copro-diagnosis of Haplorchis taichui infection using sedimentation and PCR-based methods
title_full Copro-diagnosis of Haplorchis taichui infection using sedimentation and PCR-based methods
title_fullStr Copro-diagnosis of Haplorchis taichui infection using sedimentation and PCR-based methods
title_full_unstemmed Copro-diagnosis of Haplorchis taichui infection using sedimentation and PCR-based methods
title_sort copro-diagnosis of haplorchis taichui infection using sedimentation and pcr-based methods
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=70349829696&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/59803
_version_ 1681425318906167296

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