Simple method for screening of α-thalassaemia 1 carriers

α-Thalassaemia 1 genetic disorder occurs when there is a deletion of two linked α-globin genes. The interaction between these abnormal genes leads to the most severe type of thalassaemia disease, haemoglobin (Hb) Bart's hydrops fetalis. The identification of α-thalassaemia 1 carriers and geneti...

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Main Authors: Chatchai Tayapiwatana, Surakit Kuntaruk, Thanusak Tatu, Sawitree Chiampanichayakul, Thongperm Munkongdee, Pranee Winichagoon, Suthat Fuchareon, Watchara Kasinrerk
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Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/59833
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-598332018-09-10T03:22:10Z Simple method for screening of α-thalassaemia 1 carriers Chatchai Tayapiwatana Surakit Kuntaruk Thanusak Tatu Sawitree Chiampanichayakul Thongperm Munkongdee Pranee Winichagoon Suthat Fuchareon Watchara Kasinrerk Medicine α-Thalassaemia 1 genetic disorder occurs when there is a deletion of two linked α-globin genes. The interaction between these abnormal genes leads to the most severe type of thalassaemia disease, haemoglobin (Hb) Bart's hydrops fetalis. The identification of α-thalassaemia 1 carriers and genetic counselling are essential for the prevention and control of severe thalassaemia diseases. In this study, we have developed a rapid screening method for identifying α-thalassaemia 1. A sandwich-type immunochromatographic (IC) strip test was developed, using the generated monoclonal anti-Hb Bart's antibody, to trace the Hb Bart's in haemolysates. When assayed by our IC strip test, all α-thalassaemia 1, HbH disease, HbH-Constant Spring (H-CS) disease, HbH-CS and heterozygous HbE (CSEA) Bart's disease, and homozygous α-thalassaemia 2 showed positive results. No false negative results were observed in these blood samples. In α-thalassaemia 2 heterozygotes, 83% of them showed positive reactivity. Among HbE (both homozygotes and heterozygotes), β-thalassaemia (heterozygotes, homozygotes and β-thalassaemia/HbE) and normal subjects, the IC strip test revealed negative reactivity of 100, 85 and 97%, respectively. These results indicate that this novel immunodiagnostic kit, in combination with red blood cell indices, is suitable for screening and ruling out mass populations for the presence of α-thalassaemia 1. © 2009 The Japanese Society of Hematology. 2018-09-10T03:22:10Z 2018-09-10T03:22:10Z 2009-06-01 Journal 09255710 2-s2.0-67749088035 10.1007/s12185-009-0331-4 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=67749088035&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/59833
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Medicine
spellingShingle Medicine
Chatchai Tayapiwatana
Surakit Kuntaruk
Thanusak Tatu
Sawitree Chiampanichayakul
Thongperm Munkongdee
Pranee Winichagoon
Suthat Fuchareon
Watchara Kasinrerk
Simple method for screening of α-thalassaemia 1 carriers
description α-Thalassaemia 1 genetic disorder occurs when there is a deletion of two linked α-globin genes. The interaction between these abnormal genes leads to the most severe type of thalassaemia disease, haemoglobin (Hb) Bart's hydrops fetalis. The identification of α-thalassaemia 1 carriers and genetic counselling are essential for the prevention and control of severe thalassaemia diseases. In this study, we have developed a rapid screening method for identifying α-thalassaemia 1. A sandwich-type immunochromatographic (IC) strip test was developed, using the generated monoclonal anti-Hb Bart's antibody, to trace the Hb Bart's in haemolysates. When assayed by our IC strip test, all α-thalassaemia 1, HbH disease, HbH-Constant Spring (H-CS) disease, HbH-CS and heterozygous HbE (CSEA) Bart's disease, and homozygous α-thalassaemia 2 showed positive results. No false negative results were observed in these blood samples. In α-thalassaemia 2 heterozygotes, 83% of them showed positive reactivity. Among HbE (both homozygotes and heterozygotes), β-thalassaemia (heterozygotes, homozygotes and β-thalassaemia/HbE) and normal subjects, the IC strip test revealed negative reactivity of 100, 85 and 97%, respectively. These results indicate that this novel immunodiagnostic kit, in combination with red blood cell indices, is suitable for screening and ruling out mass populations for the presence of α-thalassaemia 1. © 2009 The Japanese Society of Hematology.
format Journal
author Chatchai Tayapiwatana
Surakit Kuntaruk
Thanusak Tatu
Sawitree Chiampanichayakul
Thongperm Munkongdee
Pranee Winichagoon
Suthat Fuchareon
Watchara Kasinrerk
author_facet Chatchai Tayapiwatana
Surakit Kuntaruk
Thanusak Tatu
Sawitree Chiampanichayakul
Thongperm Munkongdee
Pranee Winichagoon
Suthat Fuchareon
Watchara Kasinrerk
author_sort Chatchai Tayapiwatana
title Simple method for screening of α-thalassaemia 1 carriers
title_short Simple method for screening of α-thalassaemia 1 carriers
title_full Simple method for screening of α-thalassaemia 1 carriers
title_fullStr Simple method for screening of α-thalassaemia 1 carriers
title_full_unstemmed Simple method for screening of α-thalassaemia 1 carriers
title_sort simple method for screening of α-thalassaemia 1 carriers
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=67749088035&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/59833
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