HPLC determination of mangostin and its application to storage stability study

A reverse phase high performance liquid chromatography (RP-HPLC) was developed and validated for the determination of mangostin in mangosteen rind crude extract and throat spray preparation. The column was a C-18 analytical column, the mobile phase consisted of methanol-water (95:5%v/v), flow rate 1...

Full description

Saved in:
Bibliographic Details
Main Authors: Pathom Jujun, Krisana Pootakham, Yanee Pongpaibul, Prasit Tharavichitkul, Chadarat Ampasavate
Format: Journal
Published: 2018
Subjects:
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=70350076118&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/60000
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Chiang Mai University
id th-cmuir.6653943832-60000
record_format dspace
spelling th-cmuir.6653943832-600002018-09-10T03:26:18Z HPLC determination of mangostin and its application to storage stability study Pathom Jujun Krisana Pootakham Yanee Pongpaibul Prasit Tharavichitkul Chadarat Ampasavate Multidisciplinary A reverse phase high performance liquid chromatography (RP-HPLC) was developed and validated for the determination of mangostin in mangosteen rind crude extract and throat spray preparation. The column was a C-18 analytical column, the mobile phase consisted of methanol-water (95:5%v/v), flow rate 1.5 ml/min and UV detector was set at 319 nm. The resulting chromatograms showed good resolution with a short retention time without interfering peak. Standard curves were constructed in the concentration range of 25-125 μg/ml (R2>0.998). The percentage recoveries at 3 levels of mangostin addition (30, 50 and 70 μg/ml) were 94.73 to 98.39% for the crude extract and 94.14 to 99.87% for the throat spray with RSDs below 2% (n=5) in all analyzed concentrations. Stability of mangostin in the throat spray containing mangosteen rind extract was also investigated by keeping the products at 4°C, 30°C, 40°C and room temperature for 180 days. The throat spray samples were found to be quite stable up to 180 days at all tested conditions. The validated HPLC method for determination of mangostin in crude mangosteen rind extract and throat spray was simple, rapid, selective and should be suitable for the quality control of mangosteen rind hydroalcoholic crude extract and antibacterial throat sprays. 2018-09-10T03:26:18Z 2018-09-10T03:26:18Z 2009-01-01 Journal 16851994 2-s2.0-70350076118 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=70350076118&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/60000
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Multidisciplinary
spellingShingle Multidisciplinary
Pathom Jujun
Krisana Pootakham
Yanee Pongpaibul
Prasit Tharavichitkul
Chadarat Ampasavate
HPLC determination of mangostin and its application to storage stability study
description A reverse phase high performance liquid chromatography (RP-HPLC) was developed and validated for the determination of mangostin in mangosteen rind crude extract and throat spray preparation. The column was a C-18 analytical column, the mobile phase consisted of methanol-water (95:5%v/v), flow rate 1.5 ml/min and UV detector was set at 319 nm. The resulting chromatograms showed good resolution with a short retention time without interfering peak. Standard curves were constructed in the concentration range of 25-125 μg/ml (R2>0.998). The percentage recoveries at 3 levels of mangostin addition (30, 50 and 70 μg/ml) were 94.73 to 98.39% for the crude extract and 94.14 to 99.87% for the throat spray with RSDs below 2% (n=5) in all analyzed concentrations. Stability of mangostin in the throat spray containing mangosteen rind extract was also investigated by keeping the products at 4°C, 30°C, 40°C and room temperature for 180 days. The throat spray samples were found to be quite stable up to 180 days at all tested conditions. The validated HPLC method for determination of mangostin in crude mangosteen rind extract and throat spray was simple, rapid, selective and should be suitable for the quality control of mangosteen rind hydroalcoholic crude extract and antibacterial throat sprays.
format Journal
author Pathom Jujun
Krisana Pootakham
Yanee Pongpaibul
Prasit Tharavichitkul
Chadarat Ampasavate
author_facet Pathom Jujun
Krisana Pootakham
Yanee Pongpaibul
Prasit Tharavichitkul
Chadarat Ampasavate
author_sort Pathom Jujun
title HPLC determination of mangostin and its application to storage stability study
title_short HPLC determination of mangostin and its application to storage stability study
title_full HPLC determination of mangostin and its application to storage stability study
title_fullStr HPLC determination of mangostin and its application to storage stability study
title_full_unstemmed HPLC determination of mangostin and its application to storage stability study
title_sort hplc determination of mangostin and its application to storage stability study
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=70350076118&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/60000
_version_ 1681425355109302272