Detection of DNA hypomethylation mediated floral induction in Longan and Spinach using the HAT-RAPD technique

Detection of DNA hypomethylation mediated floral induction in Longan and Spinach using the HAT-RAPD technique

In this study, the HAT-RAPD technique was used to detect DNA methylation in the four plant species, rice (Oryza sativa), petunia (Petunia hybrida), spinach (Spinacea oleracea L.) and longan (Dimocarpus longan Lour.), which were treated using 5-azacytidine, potassium chlorate (KClO3) and a low temper...

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Main Authors: S. Anuntalabhochai, R. Chundet, N. Buapong, R. W. Cutler
Format: Journal
Published: 2018
Subjects:
Multidisciplinary
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=52949092246&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/60009
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-600092018-09-10T03:26:23Z Detection of DNA hypomethylation mediated floral induction in Longan and Spinach using the HAT-RAPD technique S. Anuntalabhochai R. Chundet N. Buapong R. W. Cutler Multidisciplinary In this study, the HAT-RAPD technique was used to detect DNA methylation in the four plant species, rice (Oryza sativa), petunia (Petunia hybrida), spinach (Spinacea oleracea L.) and longan (Dimocarpus longan Lour.), which were treated using 5-azacytidine, potassium chlorate (KClO3) and a low temperature induction. Rice and petunia were chosen to be induced because in these species it is known that 5-azacytidine is able to induce hypomethylation in their genomes leading to morphological changes, in particular floral induction, in the developing plants. Using the HAT-RAPD technique, DNA methylation was detected using the restriction enzymes HpaII and MspI in rice and petunia (as expected) and in spinach, but was found to be absent in longan, which suggests that floral induction in longan is undetectable in longan. © 2009 Science Publications. 2018-09-10T03:26:23Z 2018-09-10T03:26:23Z 2009-01-01 Journal 15543641 15469239 2-s2.0-52949092246 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=52949092246&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/60009
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Multidisciplinary
spellingShingle Multidisciplinary
S. Anuntalabhochai
R. Chundet
N. Buapong
R. W. Cutler
Detection of DNA hypomethylation mediated floral induction in Longan and Spinach using the HAT-RAPD technique
description In this study, the HAT-RAPD technique was used to detect DNA methylation in the four plant species, rice (Oryza sativa), petunia (Petunia hybrida), spinach (Spinacea oleracea L.) and longan (Dimocarpus longan Lour.), which were treated using 5-azacytidine, potassium chlorate (KClO3) and a low temperature induction. Rice and petunia were chosen to be induced because in these species it is known that 5-azacytidine is able to induce hypomethylation in their genomes leading to morphological changes, in particular floral induction, in the developing plants. Using the HAT-RAPD technique, DNA methylation was detected using the restriction enzymes HpaII and MspI in rice and petunia (as expected) and in spinach, but was found to be absent in longan, which suggests that floral induction in longan is undetectable in longan. © 2009 Science Publications.
format Journal
author S. Anuntalabhochai
R. Chundet
N. Buapong
R. W. Cutler
author_facet S. Anuntalabhochai
R. Chundet
N. Buapong
R. W. Cutler
author_sort S. Anuntalabhochai
title Detection of DNA hypomethylation mediated floral induction in Longan and Spinach using the HAT-RAPD technique
title_short Detection of DNA hypomethylation mediated floral induction in Longan and Spinach using the HAT-RAPD technique
title_full Detection of DNA hypomethylation mediated floral induction in Longan and Spinach using the HAT-RAPD technique
title_fullStr Detection of DNA hypomethylation mediated floral induction in Longan and Spinach using the HAT-RAPD technique
title_full_unstemmed Detection of DNA hypomethylation mediated floral induction in Longan and Spinach using the HAT-RAPD technique
title_sort detection of dna hypomethylation mediated floral induction in longan and spinach using the hat-rapd technique
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=52949092246&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/60009
_version_ 1681425356791218176

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