Analysis of genetic relationship of the genus Phalaenopsis by RAPD technique

Analysis of genetic relationship of the genus Phalaenopsis by RAPD technique

Genetic relationship of 36 samples from 32 species of the genus Phalaenopsis and related genera was evaluated based on RAPD technique using 20 decamer primers. Six primers that were OPAK 10, OPD 03, OPF 01, OPF 02, OPF 09 and OPF 14 showed polymorphic DNA bands with high resolution and produced 78 p...

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Main Authors: P. Taywiya, W. Bundithya, N. Potapohn
Format: Book Series
Published: 2018
Subjects:
Agricultural and Biological Sciences
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=61449093941&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/60022
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-600222018-09-10T03:37:21Z Analysis of genetic relationship of the genus Phalaenopsis by RAPD technique P. Taywiya W. Bundithya N. Potapohn Agricultural and Biological Sciences Genetic relationship of 36 samples from 32 species of the genus Phalaenopsis and related genera was evaluated based on RAPD technique using 20 decamer primers. Six primers that were OPAK 10, OPD 03, OPF 01, OPF 02, OPF 09 and OPF 14 showed polymorphic DNA bands with high resolution and produced 78 polymorphic bands ranged in size from 223 to 2,300 bp. Genetic relationship was determined by UPGMA cluster analysis, using the Program NTSYS-PC version 2.01. Cluster analysis of six primer combinations could distinguish and divided the genus Phalaenopsis and related genera into 11 groups, which were correspondent to the analysis of genus Phalaenopsis by morphological characteristics. The genera Doritis and Kingidium were genetically distant from the genus Phalaenopsis. 2018-09-10T03:37:21Z 2018-09-10T03:37:21Z 2008-12-01 Book Series 05677572 2-s2.0-61449093941 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=61449093941&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/60022
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Agricultural and Biological Sciences
spellingShingle Agricultural and Biological Sciences
P. Taywiya
W. Bundithya
N. Potapohn
Analysis of genetic relationship of the genus Phalaenopsis by RAPD technique
description Genetic relationship of 36 samples from 32 species of the genus Phalaenopsis and related genera was evaluated based on RAPD technique using 20 decamer primers. Six primers that were OPAK 10, OPD 03, OPF 01, OPF 02, OPF 09 and OPF 14 showed polymorphic DNA bands with high resolution and produced 78 polymorphic bands ranged in size from 223 to 2,300 bp. Genetic relationship was determined by UPGMA cluster analysis, using the Program NTSYS-PC version 2.01. Cluster analysis of six primer combinations could distinguish and divided the genus Phalaenopsis and related genera into 11 groups, which were correspondent to the analysis of genus Phalaenopsis by morphological characteristics. The genera Doritis and Kingidium were genetically distant from the genus Phalaenopsis.
format Book Series
author P. Taywiya
W. Bundithya
N. Potapohn
author_facet P. Taywiya
W. Bundithya
N. Potapohn
author_sort P. Taywiya
title Analysis of genetic relationship of the genus Phalaenopsis by RAPD technique
title_short Analysis of genetic relationship of the genus Phalaenopsis by RAPD technique
title_full Analysis of genetic relationship of the genus Phalaenopsis by RAPD technique
title_fullStr Analysis of genetic relationship of the genus Phalaenopsis by RAPD technique
title_full_unstemmed Analysis of genetic relationship of the genus Phalaenopsis by RAPD technique
title_sort analysis of genetic relationship of the genus phalaenopsis by rapd technique
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=61449093941&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/60022
_version_ 1681425359193505792

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