Comparative study on methyl orange removal by growing cells and washed cell suspensions of Lactobacillus casei TISTR 1500

• Main Authors: Seesuriyachan P., Kuntiya A., Sasaki K., Techapun C.
• Format: Article
• Language: English
• Published: 2014
• Online Access: http://www.scopus.com/inward/record.url?eid=2-s2.0-67349276650&partnerID=40&md5=d9ba80be64e7e28845052d3b64b2a93a; http://cmuir.cmu.ac.th/handle/6653943832/601
• Institution: Chiang Mai University

Lactobacillus casei TISTR 1500 possesses cytoplasmic azoreductase, and converts methyl orange to N,N-dimethyl-p-phenylenediamine and 4-aminobenzenesulfonic acid. In culture growth, the strain completely degraded methyl orange at 200 mg/l, even though the pH value was lower than 4. The decolorization was inhibited in the growing culture with 800 mg of the dye/l after incubation for 12 h. The percentage of decolorization and specific decolorization rate with 400 and 800 mg/l were 66 and 15%, and 14.2, and 8.7 mg/gCell/h, respectively. Additionally, a growing culture is more tolerant to a high initial dye concentration than when using washed cell suspensions supplied with only sucrose. Moreover, incubation of a low cell density in 600 μM of Na+ and 20 mM of sucrose increased the specific decolorization rate from 2.34 mg/gCell/h (without Na+) to 4.32 mg/gCell/h. However, Na+ had no effect on the enhancement of azoreductase activity in the reaction mixture. © 2009 Springer Science+Business Media B.V.