Genomic analysis of detoxification genes in the mosquito Aedes aegypti
Annotation of the recently determined genome sequence of the major dengue vector, Aedes aegypti, reveals an abundance of detoxification genes. Here, we report the presence of 235 members of the cytochrome P450, glutathione transferase and carboxy/cholinesterase families in Ae. aegypti. This gene cou...
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th-cmuir.6653943832-601322018-09-10T03:39:23Z Genomic analysis of detoxification genes in the mosquito Aedes aegypti Clare Strode Charles S. Wondji Jean Philippe David Nicola J. Hawkes Nongkran Lumjuan David R. Nelson David R. Drane S. H P Parakrama Karunaratne Janet Hemingway William C. Black IV Hilary Ranson Agricultural and Biological Sciences Biochemistry, Genetics and Molecular Biology Annotation of the recently determined genome sequence of the major dengue vector, Aedes aegypti, reveals an abundance of detoxification genes. Here, we report the presence of 235 members of the cytochrome P450, glutathione transferase and carboxy/cholinesterase families in Ae. aegypti. This gene count represents an increase of 58% and 36% compared with the fruitfly, Drosophila melanogaster, and the malaria mosquito, Anopheles gambiae, respectively. The expansion is not uniform within the gene families. Secure orthologs can be found across the insect species for enzymes that have presumed or proven biosynthetic or housekeeping roles. In contrast, subsets of these gene families that are associated with general xenobiotic detoxification, in particular the CYP6, CYP9 and alpha esterase families, have expanded in Ae. aegypti. In order to identify detoxification genes associated with resistance to insecticides we constructed an array containing unique oligonucleotide probes for these genes and compared their expression level in insecticide resistant and susceptible strains. Several candidate genes were identified with the majority belonging to two gene families, the CYP9 P450s and the Epsilon GSTs. This 'Ae. aegypti Detox Chip' will facilitate the implementation of insecticide resistance management strategies for arboviral control programmes. © 2007 Elsevier Ltd. All rights reserved. 2018-09-10T03:38:26Z 2018-09-10T03:38:26Z 2008-01-01 Journal 09651748 2-s2.0-36749069363 10.1016/j.ibmb.2007.09.007 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=36749069363&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/60132 |
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Agricultural and Biological Sciences Biochemistry, Genetics and Molecular Biology Clare Strode Charles S. Wondji Jean Philippe David Nicola J. Hawkes Nongkran Lumjuan David R. Nelson David R. Drane S. H P Parakrama Karunaratne Janet Hemingway William C. Black IV Hilary Ranson Genomic analysis of detoxification genes in the mosquito Aedes aegypti |
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Annotation of the recently determined genome sequence of the major dengue vector, Aedes aegypti, reveals an abundance of detoxification genes. Here, we report the presence of 235 members of the cytochrome P450, glutathione transferase and carboxy/cholinesterase families in Ae. aegypti. This gene count represents an increase of 58% and 36% compared with the fruitfly, Drosophila melanogaster, and the malaria mosquito, Anopheles gambiae, respectively. The expansion is not uniform within the gene families. Secure orthologs can be found across the insect species for enzymes that have presumed or proven biosynthetic or housekeeping roles. In contrast, subsets of these gene families that are associated with general xenobiotic detoxification, in particular the CYP6, CYP9 and alpha esterase families, have expanded in Ae. aegypti. In order to identify detoxification genes associated with resistance to insecticides we constructed an array containing unique oligonucleotide probes for these genes and compared their expression level in insecticide resistant and susceptible strains. Several candidate genes were identified with the majority belonging to two gene families, the CYP9 P450s and the Epsilon GSTs. This 'Ae. aegypti Detox Chip' will facilitate the implementation of insecticide resistance management strategies for arboviral control programmes. © 2007 Elsevier Ltd. All rights reserved. |
format |
Journal |
author |
Clare Strode Charles S. Wondji Jean Philippe David Nicola J. Hawkes Nongkran Lumjuan David R. Nelson David R. Drane S. H P Parakrama Karunaratne Janet Hemingway William C. Black IV Hilary Ranson |
author_facet |
Clare Strode Charles S. Wondji Jean Philippe David Nicola J. Hawkes Nongkran Lumjuan David R. Nelson David R. Drane S. H P Parakrama Karunaratne Janet Hemingway William C. Black IV Hilary Ranson |
author_sort |
Clare Strode |
title |
Genomic analysis of detoxification genes in the mosquito Aedes aegypti |
title_short |
Genomic analysis of detoxification genes in the mosquito Aedes aegypti |
title_full |
Genomic analysis of detoxification genes in the mosquito Aedes aegypti |
title_fullStr |
Genomic analysis of detoxification genes in the mosquito Aedes aegypti |
title_full_unstemmed |
Genomic analysis of detoxification genes in the mosquito Aedes aegypti |
title_sort |
genomic analysis of detoxification genes in the mosquito aedes aegypti |
publishDate |
2018 |
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https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=36749069363&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/60132 |
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