Cloning and expression of chitinase A gene from Streptomyces cyaneus SP-27: The enzyme participates in protoplast formation of Schizophyllum commune

• Main Authors: Shigekazu Yano, Arata Honda, Nopakarn Rattanakit, Yuta Noda, Mamoru Wakayama, Abhinya Plikomol, Takashi Tachiki
• Format: Journal
• Published: 2018
• Subjects: Biochemistry, Genetics and Molecular Biology; Chemistry; Immunology and Microbiology
• Online Access: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=47949114147&origin=inward; http://cmuir.cmu.ac.th/jspui/handle/6653943832/60158
• Institution: Chiang Mai University

Chitinase A of Streptomyces cyaneus SP-27 or chitinase I of Bacillus circulans KA-304 showed the protoplast-forming activity when combined with α-1,3-glucanase of B. circulans KA-304. The gene of chitinase A was cloned. It consisted of 903 nucleotides encoding 301 amino acid residues, including a pu...