Generation of functional scFv intrabody to abate the expression of CD147 surface molecule of 293A cells

• Main Authors: Khajornsak Tragoolpua, Nutjeera Intasai, Watchara Kasinrerk, Sabine Mai, Yuan Yuan, Chatchai Tayapiwatana
• Format: Journal
• Published: 2018
• Subjects: Biochemistry, Genetics and Molecular Biology
• Online Access: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=39849084770&origin=inward; http://cmuir.cmu.ac.th/jspui/handle/6653943832/60186
• Institution: Chiang Mai University

Background: Expression of intracellular antibodies (intrabodies) has become a broadly applicable technology for generation of phenotypic knockouts in vivo. The method uses surface depletion of cellular membrane proteins to examine their biological function. In this study, we used this strategy to bl...