Enhancement of androstadienedione production from progesterone by biotransformation using the hydroxypropyl-β-cyclodextrin complexation technique

The enhanced production of androstadienedione (ADD) from progesterone (P) using the hydroxypropyl-β-cyclodextrin (HPβCD) complexation technique by biotransformation was demonstrated. The microorganisms used were Bacillus sphaericus ATCC 245, B. sphaericus ATCC 7063, B. sphaericus ATCC 13805, Arthrob...

Full description

Saved in:
Bibliographic Details
Main Authors: Aranya Manosroi, Suda Saowakhon, Jiradej Manosroi
Format: Journal
Published: 2018
Subjects:
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=38649103108&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/60203
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Chiang Mai University
id th-cmuir.6653943832-60203
record_format dspace
spelling th-cmuir.6653943832-602032018-09-10T03:47:20Z Enhancement of androstadienedione production from progesterone by biotransformation using the hydroxypropyl-β-cyclodextrin complexation technique Aranya Manosroi Suda Saowakhon Jiradej Manosroi Biochemistry, Genetics and Molecular Biology Medicine The enhanced production of androstadienedione (ADD) from progesterone (P) using the hydroxypropyl-β-cyclodextrin (HPβCD) complexation technique by biotransformation was demonstrated. The microorganisms used were Bacillus sphaericus ATCC 245, B. sphaericus ATCC 7063, B. sphaericus ATCC 13805, Arthrobacter simplex ATCC 6946, B. sphaericus TISTR 670 and those screened from soils in Chiang Mai Province, Thailand which were B. sphaericus SRP I, B. sphaericus SRP II and B. sphaericus SRP III. The complexed (P-complex) and the uncomplexed P at 0.3-1.2 mg/ml were investigated. Samples were withdrawn from the bioconversion mixture at various time intervals for 168 h. The ADD and P contents were determined by HPLC. All organisms showed ADD production from either P or P-complex by one-step biotransformation (including side chain cleavage and dehydrogenation). At 0.3 mg/ml of P in the systems of B. sphaericus ATCC 13805, A. simplex ATCC 6946 and B. sphaericus ATCC 245, the uncomplexed form showed the highest ADD yield of 2.82, 1.63 and 64.67% at 168, 168 and 144 h, whereas P-complex gave 98.44, 19.58 and 97.10% at 144, 24 and 168 h, respectively. This indicated an increase of ADD production from the P-complex in comparison to P of 35, 12 and 1.5 times, respectively. This study has shown that the complexation of P with HPβCD enhanced the ADD production in a novel one-step bioconversion. © 2007 Elsevier Ltd. All rights reserved. 2018-09-10T03:39:18Z 2018-09-10T03:39:18Z 2008-01-01 Journal 09600760 2-s2.0-38649103108 10.1016/j.jsbmb.2007.05.032 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=38649103108&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/60203
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
Medicine
spellingShingle Biochemistry, Genetics and Molecular Biology
Medicine
Aranya Manosroi
Suda Saowakhon
Jiradej Manosroi
Enhancement of androstadienedione production from progesterone by biotransformation using the hydroxypropyl-β-cyclodextrin complexation technique
description The enhanced production of androstadienedione (ADD) from progesterone (P) using the hydroxypropyl-β-cyclodextrin (HPβCD) complexation technique by biotransformation was demonstrated. The microorganisms used were Bacillus sphaericus ATCC 245, B. sphaericus ATCC 7063, B. sphaericus ATCC 13805, Arthrobacter simplex ATCC 6946, B. sphaericus TISTR 670 and those screened from soils in Chiang Mai Province, Thailand which were B. sphaericus SRP I, B. sphaericus SRP II and B. sphaericus SRP III. The complexed (P-complex) and the uncomplexed P at 0.3-1.2 mg/ml were investigated. Samples were withdrawn from the bioconversion mixture at various time intervals for 168 h. The ADD and P contents were determined by HPLC. All organisms showed ADD production from either P or P-complex by one-step biotransformation (including side chain cleavage and dehydrogenation). At 0.3 mg/ml of P in the systems of B. sphaericus ATCC 13805, A. simplex ATCC 6946 and B. sphaericus ATCC 245, the uncomplexed form showed the highest ADD yield of 2.82, 1.63 and 64.67% at 168, 168 and 144 h, whereas P-complex gave 98.44, 19.58 and 97.10% at 144, 24 and 168 h, respectively. This indicated an increase of ADD production from the P-complex in comparison to P of 35, 12 and 1.5 times, respectively. This study has shown that the complexation of P with HPβCD enhanced the ADD production in a novel one-step bioconversion. © 2007 Elsevier Ltd. All rights reserved.
format Journal
author Aranya Manosroi
Suda Saowakhon
Jiradej Manosroi
author_facet Aranya Manosroi
Suda Saowakhon
Jiradej Manosroi
author_sort Aranya Manosroi
title Enhancement of androstadienedione production from progesterone by biotransformation using the hydroxypropyl-β-cyclodextrin complexation technique
title_short Enhancement of androstadienedione production from progesterone by biotransformation using the hydroxypropyl-β-cyclodextrin complexation technique
title_full Enhancement of androstadienedione production from progesterone by biotransformation using the hydroxypropyl-β-cyclodextrin complexation technique
title_fullStr Enhancement of androstadienedione production from progesterone by biotransformation using the hydroxypropyl-β-cyclodextrin complexation technique
title_full_unstemmed Enhancement of androstadienedione production from progesterone by biotransformation using the hydroxypropyl-β-cyclodextrin complexation technique
title_sort enhancement of androstadienedione production from progesterone by biotransformation using the hydroxypropyl-β-cyclodextrin complexation technique
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=38649103108&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/60203
_version_ 1681425392547659776