Basidiome formation of an edible wild, putatively ectomycorrhizal fungus, Phlebopus portentosus without host plant
Phlebopus portentosus is a popular wild edible ectomycorrhizal fungus in northern Thailand. In general ectomycorrhizal fungi produce basidiomes when associated with a host plant. In this paper mycelium growth and basidiome production of P. portentosus were examined in pure culture both in vitro and...
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th-cmuir.6653943832-60382014-08-30T03:23:46Z Basidiome formation of an edible wild, putatively ectomycorrhizal fungus, Phlebopus portentosus without host plant Kumla J. Bussaban B. Suwannarach N. Lumyong S. Danell E. Phlebopus portentosus is a popular wild edible ectomycorrhizal fungus in northern Thailand. In general ectomycorrhizal fungi produce basidiomes when associated with a host plant. In this paper mycelium growth and basidiome production of P. portentosus were examined in pure culture both in vitro and in pot-culture experiments. Five mycelial strains of P. portentosus were isolated from basidiomes and used in the experiments. The mycelia grew fastest on sorghum grains supplemented with fungal-host solution. The mycelia produced sclerotia-like structures after 3 wk incubation in darkness at 30 C. All strains of P. portentosus had the ability to form primordia. The primordia were formed under lowered temperature, high humidity and a 12 h photoperiod. They developed to mature basidiomes after 8-12 d in in vitro. In the pot-culture primordia were found after 28-35 d incubation in the greenhouse and mature basidiomes released basidiospores within 6-8 d. Basidiospores were germinated on fungal-host medium and formed mycelial colonies. This fungus showed an ability to produce basidiomes even 2 y after the original isolation from tissues. This research provides valuable information concerning the techniques and protocols for the large scale commercial production of P. portentosus basidiomes in the absence of a host plant. © 2012 by The Mycological Society of America. 2014-08-30T03:23:46Z 2014-08-30T03:23:46Z 2012 Article 275514 10.3852/11-074 MYCOA http://www.ncbi.nlm.nih.gov/pubmed/3502482 http://www.scopus.com/inward/record.url?eid=2-s2.0-84863535014&partnerID=40&md5=e0aa1a646d45d51de5c549afd45bf760 http://cmuir.cmu.ac.th/handle/6653943832/6038 English |
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Phlebopus portentosus is a popular wild edible ectomycorrhizal fungus in northern Thailand. In general ectomycorrhizal fungi produce basidiomes when associated with a host plant. In this paper mycelium growth and basidiome production of P. portentosus were examined in pure culture both in vitro and in pot-culture experiments. Five mycelial strains of P. portentosus were isolated from basidiomes and used in the experiments. The mycelia grew fastest on sorghum grains supplemented with fungal-host solution. The mycelia produced sclerotia-like structures after 3 wk incubation in darkness at 30 C. All strains of P. portentosus had the ability to form primordia. The primordia were formed under lowered temperature, high humidity and a 12 h photoperiod. They developed to mature basidiomes after 8-12 d in in vitro. In the pot-culture primordia were found after 28-35 d incubation in the greenhouse and mature basidiomes released basidiospores within 6-8 d. Basidiospores were germinated on fungal-host medium and formed mycelial colonies. This fungus showed an ability to produce basidiomes even 2 y after the original isolation from tissues. This research provides valuable information concerning the techniques and protocols for the large scale commercial production of P. portentosus basidiomes in the absence of a host plant. © 2012 by The Mycological Society of America. |
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Kumla J. Bussaban B. Suwannarach N. Lumyong S. Danell E. |
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Kumla J. Bussaban B. Suwannarach N. Lumyong S. Danell E. Basidiome formation of an edible wild, putatively ectomycorrhizal fungus, Phlebopus portentosus without host plant |
author_facet |
Kumla J. Bussaban B. Suwannarach N. Lumyong S. Danell E. |
author_sort |
Kumla J. |
title |
Basidiome formation of an edible wild, putatively ectomycorrhizal fungus, Phlebopus portentosus without host plant |
title_short |
Basidiome formation of an edible wild, putatively ectomycorrhizal fungus, Phlebopus portentosus without host plant |
title_full |
Basidiome formation of an edible wild, putatively ectomycorrhizal fungus, Phlebopus portentosus without host plant |
title_fullStr |
Basidiome formation of an edible wild, putatively ectomycorrhizal fungus, Phlebopus portentosus without host plant |
title_full_unstemmed |
Basidiome formation of an edible wild, putatively ectomycorrhizal fungus, Phlebopus portentosus without host plant |
title_sort |
basidiome formation of an edible wild, putatively ectomycorrhizal fungus, phlebopus portentosus without host plant |
publishDate |
2014 |
url |
http://www.ncbi.nlm.nih.gov/pubmed/3502482 http://www.scopus.com/inward/record.url?eid=2-s2.0-84863535014&partnerID=40&md5=e0aa1a646d45d51de5c549afd45bf760 http://cmuir.cmu.ac.th/handle/6653943832/6038 |
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