Rapid detection of Brugia malayi in mosquito vectors using a real-time fluorescence resonance energy transfer PCR and melting curve analysis

• Main Authors: Tongjit Thanchomnang, Pewpan M. Intapan, Viraphong Lulitanond, Wej Choochote, Anonglak Manjai, Thidarat K. Prasongdee, Wanchai Maleewong
• Format: Journal
• Published: 2018
• Subjects: Immunology and Microbiology; Medicine
• Online Access: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=42949097929&origin=inward; http://cmuir.cmu.ac.th/jspui/handle/6653943832/60481
• Institution: Chiang Mai University

We developed real-time fluorescence resonance energy transfer (FRET) polymerase chain reaction (PCR) combined with melting curve analysis for detection of Brugia malayi DNA in blood-fed mosquitoes. Real-time FRET PCR is based on a fluorescence melting curve analysis of hybrid formed between amplicon...