Multiple combinations of P[13]-like genotype with G3, G4, and G5 in porcine rotaviruses

Epidemiological surveillance of porcine rotavirus (PoRV) strains was carried out in Chiang Mai Province, Thailand, from 2002 to 2003, and eight rotavirus isolates could not be completely typed by PCR. Of these, six were G3 and one was G4 and displayed a P-nontypeable genotype, while another isolate...

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Main Authors: Wisoot Chan-it, Pattara Khamrin, Prayuth Saekhow, Chansom Pantip, Aksara Thongprachum, Supatra Peerakome, Hiroshi Ushijima, Niwat Maneekarn
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/60659
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spelling th-cmuir.6653943832-606592018-09-10T03:46:46Z Multiple combinations of P[13]-like genotype with G3, G4, and G5 in porcine rotaviruses Wisoot Chan-it Pattara Khamrin Prayuth Saekhow Chansom Pantip Aksara Thongprachum Supatra Peerakome Hiroshi Ushijima Niwat Maneekarn Medicine Epidemiological surveillance of porcine rotavirus (PoRV) strains was carried out in Chiang Mai Province, Thailand, from 2002 to 2003, and eight rotavirus isolates could not be completely typed by PCR. Of these, six were G3 and one was G4 and displayed a P-nontypeable genotype, while another isolate was both G and P nontypeable. Analysis of a partial VP4 gene of all eight P-nontypeable strains revealed a high degree of amino acid sequence identities (94.7% to 100%), suggesting that they belonged to the same P genotype. Comparison of the amino acid sequences of two representative strains (namely, strains CMP178 and CMP213) with those of 27 other known P genotypes revealed a high degree of amino acid sequence identity with those of P[13] porcine rotavirus reference strains HP113 and HP140, which were recently isolated in India. However, amino acid sequence comparison with non-P[13] rotavirus strains revealed relatively low identities, ranging from 58.2% to 84.8% for full-length VP4 sequences and 35.1% to 80.6% for VP8* sequences. Phylogenetic analysis revealed that CMP178 and CMP213 clustered together in a monophyletic branch with P[13]-like genotypes HP113 and HP140 which was clearly separated from the other lineages of P[13] or P[22] strains. Altogether, these findings indicate that PoRV strains CMP178 and CMP213 should be considered the P[13]-like VP4 genotype, a rare genotype that has been identified only in pigs. This study provides additional evidence of increasing genetic diversity among group A rotaviruses in nature. Copyright © 2008, American Society for Microbiology. All Rights Reserved. 2018-09-10T03:46:46Z 2018-09-10T03:46:46Z 2008-04-01 Journal 00951137 2-s2.0-42449146173 10.1128/JCM.00856-07 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=42449146173&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/60659
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Medicine
spellingShingle Medicine
Wisoot Chan-it
Pattara Khamrin
Prayuth Saekhow
Chansom Pantip
Aksara Thongprachum
Supatra Peerakome
Hiroshi Ushijima
Niwat Maneekarn
Multiple combinations of P[13]-like genotype with G3, G4, and G5 in porcine rotaviruses
description Epidemiological surveillance of porcine rotavirus (PoRV) strains was carried out in Chiang Mai Province, Thailand, from 2002 to 2003, and eight rotavirus isolates could not be completely typed by PCR. Of these, six were G3 and one was G4 and displayed a P-nontypeable genotype, while another isolate was both G and P nontypeable. Analysis of a partial VP4 gene of all eight P-nontypeable strains revealed a high degree of amino acid sequence identities (94.7% to 100%), suggesting that they belonged to the same P genotype. Comparison of the amino acid sequences of two representative strains (namely, strains CMP178 and CMP213) with those of 27 other known P genotypes revealed a high degree of amino acid sequence identity with those of P[13] porcine rotavirus reference strains HP113 and HP140, which were recently isolated in India. However, amino acid sequence comparison with non-P[13] rotavirus strains revealed relatively low identities, ranging from 58.2% to 84.8% for full-length VP4 sequences and 35.1% to 80.6% for VP8* sequences. Phylogenetic analysis revealed that CMP178 and CMP213 clustered together in a monophyletic branch with P[13]-like genotypes HP113 and HP140 which was clearly separated from the other lineages of P[13] or P[22] strains. Altogether, these findings indicate that PoRV strains CMP178 and CMP213 should be considered the P[13]-like VP4 genotype, a rare genotype that has been identified only in pigs. This study provides additional evidence of increasing genetic diversity among group A rotaviruses in nature. Copyright © 2008, American Society for Microbiology. All Rights Reserved.
format Journal
author Wisoot Chan-it
Pattara Khamrin
Prayuth Saekhow
Chansom Pantip
Aksara Thongprachum
Supatra Peerakome
Hiroshi Ushijima
Niwat Maneekarn
author_facet Wisoot Chan-it
Pattara Khamrin
Prayuth Saekhow
Chansom Pantip
Aksara Thongprachum
Supatra Peerakome
Hiroshi Ushijima
Niwat Maneekarn
author_sort Wisoot Chan-it
title Multiple combinations of P[13]-like genotype with G3, G4, and G5 in porcine rotaviruses
title_short Multiple combinations of P[13]-like genotype with G3, G4, and G5 in porcine rotaviruses
title_full Multiple combinations of P[13]-like genotype with G3, G4, and G5 in porcine rotaviruses
title_fullStr Multiple combinations of P[13]-like genotype with G3, G4, and G5 in porcine rotaviruses
title_full_unstemmed Multiple combinations of P[13]-like genotype with G3, G4, and G5 in porcine rotaviruses
title_sort multiple combinations of p[13]-like genotype with g3, g4, and g5 in porcine rotaviruses
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=42449146173&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/60659
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