Sequential injection-capillary immunoassay system for determination of sialoglycoconjugates
An automatic immunoassay system for an assay of sialoglycoconjugates was developed based on the sequential injection technique. A cost effective plain glass capillary tube was used as a solid surface for immobilization of biomolecules via a simple physical adsorption which is adequate to tolerate th...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Evaluation Studies |
| Language: | English |
| Published: |
2014
|
| Online Access: | http://www.ncbi.nlm.nih.gov/pubmed/3502482 http://cmuir.cmu.ac.th/handle/6653943832/6067 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Institution: | Chiang Mai University |
| Language: | English |
| Summary: | An automatic immunoassay system for an assay of sialoglycoconjugates was developed based on the sequential injection technique. A cost effective plain glass capillary tube was used as a solid surface for immobilization of biomolecules via a simple physical adsorption which is adequate to tolerate the force of solution flowing through the capillary during the multi-steps immunoassay process. Immunoassay could be performed with many improvements-rapidity per sample as compared to the conventional micro-plate format (40 min vs. 5-8 h); lower cost and simpler as compared to fused silica capillary with covalent immobilization; and without problem of back pressure as compared to flow injection-bead based immunoassay. Performance of the sequential injection-capillary immunoassay was demonstrated by assay of sialoglycoconjugates level in human serum to differentiate cancer patients from healthy people. |
|---|