The Aedes aegypti glutathione transferase family

In this report, we describe the glutathione transferase (GST) gene family in the dengue vector Aedes aegypti and suggest a novel role for a new class of mosquito GSTs. Twenty-six GST genes are present in Ae. aegypti, two of which are alternatively spliced to give a total of 29 transcripts for cytoso...

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Main Authors: Nongkran Lumjuan, Bradley J. Stevenson, La aied Prapanthadara, Pradya Somboon, Peter M. Brophy, Brendan J. Loftus, David W. Severson, Hilary Ranson
Format: Journal
Published: 2018
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-607862018-09-10T04:00:49Z The Aedes aegypti glutathione transferase family Nongkran Lumjuan Bradley J. Stevenson La aied Prapanthadara Pradya Somboon Peter M. Brophy Brendan J. Loftus David W. Severson Hilary Ranson Agricultural and Biological Sciences Biochemistry, Genetics and Molecular Biology In this report, we describe the glutathione transferase (GST) gene family in the dengue vector Aedes aegypti and suggest a novel role for a new class of mosquito GSTs. Twenty-six GST genes are present in Ae. aegypti, two of which are alternatively spliced to give a total of 29 transcripts for cytosolic GSTs. The six classes identified in other insect species are all represented and, as in Anopheles gambiae, the majority of the mosquito GSTs belong to the insect-specific Delta and Epsilon classes with eight members each. Sixteen secure 1:1 orthologs were identified between GSTs in Ae. aegypti and An. gambiae, but only four of these have recognisable orthologs in Drosophila melanogaster. Three mosquito-specific GSTs were identified which did not belong to any previously recognised GST classes. One of these, GSTx2, has been previously implicated in conferring 1,1,1-trichloro-2,2-bis-(p-chlorophenyl)ethane (DDT) resistance in Ae. aegypti from South America. However, we found no evidence for increased levels of this GST protein in DDT/pyrethroid-resistant populations from Thailand. Furthermore, we show that the recombinant GSTX2-2 protein is unable to metabolise DDT. Interestingly, GSTX2-2 showed an affinity for hematin, and this, together with the restricted distribution of this class to haematophagous insects, may indicate a role for these enzymes in protecting mosquitoes against heme toxicity during blood feeding. © 2007 Elsevier Ltd. All rights reserved. 2018-09-10T03:59:39Z 2018-09-10T03:59:39Z 2007-10-01 Journal 09651748 2-s2.0-34548261707 10.1016/j.ibmb.2007.05.018 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=34548261707&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/60786
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Agricultural and Biological Sciences
Biochemistry, Genetics and Molecular Biology
spellingShingle Agricultural and Biological Sciences
Biochemistry, Genetics and Molecular Biology
Nongkran Lumjuan
Bradley J. Stevenson
La aied Prapanthadara
Pradya Somboon
Peter M. Brophy
Brendan J. Loftus
David W. Severson
Hilary Ranson
The Aedes aegypti glutathione transferase family
description In this report, we describe the glutathione transferase (GST) gene family in the dengue vector Aedes aegypti and suggest a novel role for a new class of mosquito GSTs. Twenty-six GST genes are present in Ae. aegypti, two of which are alternatively spliced to give a total of 29 transcripts for cytosolic GSTs. The six classes identified in other insect species are all represented and, as in Anopheles gambiae, the majority of the mosquito GSTs belong to the insect-specific Delta and Epsilon classes with eight members each. Sixteen secure 1:1 orthologs were identified between GSTs in Ae. aegypti and An. gambiae, but only four of these have recognisable orthologs in Drosophila melanogaster. Three mosquito-specific GSTs were identified which did not belong to any previously recognised GST classes. One of these, GSTx2, has been previously implicated in conferring 1,1,1-trichloro-2,2-bis-(p-chlorophenyl)ethane (DDT) resistance in Ae. aegypti from South America. However, we found no evidence for increased levels of this GST protein in DDT/pyrethroid-resistant populations from Thailand. Furthermore, we show that the recombinant GSTX2-2 protein is unable to metabolise DDT. Interestingly, GSTX2-2 showed an affinity for hematin, and this, together with the restricted distribution of this class to haematophagous insects, may indicate a role for these enzymes in protecting mosquitoes against heme toxicity during blood feeding. © 2007 Elsevier Ltd. All rights reserved.
format Journal
author Nongkran Lumjuan
Bradley J. Stevenson
La aied Prapanthadara
Pradya Somboon
Peter M. Brophy
Brendan J. Loftus
David W. Severson
Hilary Ranson
author_facet Nongkran Lumjuan
Bradley J. Stevenson
La aied Prapanthadara
Pradya Somboon
Peter M. Brophy
Brendan J. Loftus
David W. Severson
Hilary Ranson
author_sort Nongkran Lumjuan
title The Aedes aegypti glutathione transferase family
title_short The Aedes aegypti glutathione transferase family
title_full The Aedes aegypti glutathione transferase family
title_fullStr The Aedes aegypti glutathione transferase family
title_full_unstemmed The Aedes aegypti glutathione transferase family
title_sort aedes aegypti glutathione transferase family
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=34548261707&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/60786
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