Sensitization to Fas-mediated apoptosis by dengue virus capsid protein

• Main Authors: Thawornchai Limjindaporn, Janjuree Netsawang, Sansanee Noisakran, Somchai Thiemmeca, Wiyada Wongwiwat, Sangkab Sudsaward, Panisadee Avirutnan, Chunya Puttikhunt, Watchara Kasinrerk, Rungtawan Sriburi, Nopporn Sittisombut, Pa thai Yenchitsomanus, Prida Malasit
• Format: Journal
• Published: 2018
• Subjects: Biochemistry, Genetics and Molecular Biology
• Online Access: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=34548290826&origin=inward; http://cmuir.cmu.ac.th/jspui/handle/6653943832/60882
• Institution: Chiang Mai University

Dengue fever (DF) and dengue hemorrhagic fever (DHF) are important public health problems in tropical regions. Abnormal hemostasis and plasma leakage are the main patho-physiological changes in DHF. However, hepatomegaly, hepatocellular necrosis and fulminant hepatic failure are occasionally observed in patients with DHF. Dengue virus-infected liver cells undergo apoptosis but the underlying molecular mechanism remains unclear. Using a yeast two-hybrid screen, we found that dengue virus capsid protein (DENV C) physically interacts with the human death domain-associated protein Daxx, a Fas-associated protein. The interaction between DENV C and Daxx in dengue virus-infected liver cells was also demonstrated by co-immunoprecipitation and double immunofluorescence staining. The two proteins were predominantly co-localized in the cellular nuclei. Fas-mediated apoptotic activity in liver cells constitutively expressing DENV C was induced by anti-Fas antibody, indicating that the interaction of DENV C and Daxx involves in apoptosis of dengue virus-infected liver cells. © 2007 Elsevier Inc. All rights reserved.