Online assay of bone specific alkaline phosphatase with a flow injection-bead injection system

Alkaline phosphatase (ALP) has been used as one of the biomarkers for bone resorption and liver diseases. Normally, total alkaline phosphatase is quantified along with other symptoms to determine the releasing source of the alkaline phosphatase. A semi-automated flow injection-bead injection system...

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Main Authors: Supaporn Kradtap Hartwell, Duangporn Somprayoon, Prachya Kongtawelert, Siriwan Ongchai, Olarn Arppornchayanon, Lucksagoon Ganranoo, Somchai Lapanantnoppakhun, Kate Grudpan
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/60885
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-608852018-09-10T04:04:20Z Online assay of bone specific alkaline phosphatase with a flow injection-bead injection system Supaporn Kradtap Hartwell Duangporn Somprayoon Prachya Kongtawelert Siriwan Ongchai Olarn Arppornchayanon Lucksagoon Ganranoo Somchai Lapanantnoppakhun Kate Grudpan Biochemistry, Genetics and Molecular Biology Chemistry Environmental Science Alkaline phosphatase (ALP) has been used as one of the biomarkers for bone resorption and liver diseases. Normally, total alkaline phosphatase is quantified along with other symptoms to determine the releasing source of the alkaline phosphatase. A semi-automated flow injection-bead injection system was proposed to conveniently and selectively assay bone alkaline phosphatase (BALP) based on its specific binding to wheat germ coated beads. Amount of BALP in serum was determined from the intensity of the yellow product produced from bound BALP on the retained beads and its substrate pNPP. The used beads were discarded and the fresh ones were introduced for the next analysis. The reaction cell was designed to be opened and closed using a computer controlled solenoid valve for a precise incubation time. The performance of the proposed system was evaluated by using it to assay BALP in human serum. The results were compared to those obtained by using a commercial ELISA kit. The system is proposed to be an easy and cost effective system for quantification of BALP as an alternative to batch wise wheat germ specific binding technique. © 2007 Elsevier B.V. All rights reserved. 2018-09-10T04:00:50Z 2018-09-10T04:00:50Z 2007-09-26 Journal 00032670 2-s2.0-34548812601 10.1016/j.aca.2006.12.056 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=34548812601&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/60885
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
Chemistry
Environmental Science
spellingShingle Biochemistry, Genetics and Molecular Biology
Chemistry
Environmental Science
Supaporn Kradtap Hartwell
Duangporn Somprayoon
Prachya Kongtawelert
Siriwan Ongchai
Olarn Arppornchayanon
Lucksagoon Ganranoo
Somchai Lapanantnoppakhun
Kate Grudpan
Online assay of bone specific alkaline phosphatase with a flow injection-bead injection system
description Alkaline phosphatase (ALP) has been used as one of the biomarkers for bone resorption and liver diseases. Normally, total alkaline phosphatase is quantified along with other symptoms to determine the releasing source of the alkaline phosphatase. A semi-automated flow injection-bead injection system was proposed to conveniently and selectively assay bone alkaline phosphatase (BALP) based on its specific binding to wheat germ coated beads. Amount of BALP in serum was determined from the intensity of the yellow product produced from bound BALP on the retained beads and its substrate pNPP. The used beads were discarded and the fresh ones were introduced for the next analysis. The reaction cell was designed to be opened and closed using a computer controlled solenoid valve for a precise incubation time. The performance of the proposed system was evaluated by using it to assay BALP in human serum. The results were compared to those obtained by using a commercial ELISA kit. The system is proposed to be an easy and cost effective system for quantification of BALP as an alternative to batch wise wheat germ specific binding technique. © 2007 Elsevier B.V. All rights reserved.
format Journal
author Supaporn Kradtap Hartwell
Duangporn Somprayoon
Prachya Kongtawelert
Siriwan Ongchai
Olarn Arppornchayanon
Lucksagoon Ganranoo
Somchai Lapanantnoppakhun
Kate Grudpan
author_facet Supaporn Kradtap Hartwell
Duangporn Somprayoon
Prachya Kongtawelert
Siriwan Ongchai
Olarn Arppornchayanon
Lucksagoon Ganranoo
Somchai Lapanantnoppakhun
Kate Grudpan
author_sort Supaporn Kradtap Hartwell
title Online assay of bone specific alkaline phosphatase with a flow injection-bead injection system
title_short Online assay of bone specific alkaline phosphatase with a flow injection-bead injection system
title_full Online assay of bone specific alkaline phosphatase with a flow injection-bead injection system
title_fullStr Online assay of bone specific alkaline phosphatase with a flow injection-bead injection system
title_full_unstemmed Online assay of bone specific alkaline phosphatase with a flow injection-bead injection system
title_sort online assay of bone specific alkaline phosphatase with a flow injection-bead injection system
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=34548812601&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/60885
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