Determination of cefadroxil by sequential injection with spectrophotometric detector

A sequential injection analysis (SIA) spectrophotometric procedure for cefadroxil determination has been developed. The SIA instrumentation was modified to achieve the desired function and operations by using the software developed to interface the PC with the conventional SIA system. The method is...

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Main Authors: Makchit J., Upalee S., Thongpoon C., Liawruangrath B., Liawruangrath S.
Format: Article
Language:English
Published: 2014
Online Access:http://www.ncbi.nlm.nih.gov/pubmed/3502482
http://cmuir.cmu.ac.th/handle/6653943832/6089
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Institution: Chiang Mai University
Language: English
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spelling th-cmuir.6653943832-60892014-08-30T03:23:49Z Determination of cefadroxil by sequential injection with spectrophotometric detector Makchit J. Upalee S. Thongpoon C. Liawruangrath B. Liawruangrath S. A sequential injection analysis (SIA) spectrophotometric procedure for cefadroxil determination has been developed. The SIA instrumentation was modified to achieve the desired function and operations by using the software developed to interface the PC with the conventional SIA system. The method is based on the measurement of a red, water-soluble product formed by the reaction between cefadroxil and 4-aminoantipyrine in the presence of alkaline potassium hexacyanoferrate(III) at 510 nm. Optimum conditions for determining the drug were investigated. Beer's law was obeyed over the concentration ranges of 1 - 10 mg L(-1) and 10 - 50 mg L(-1) with a detection limit (3 sigma) of 0.17 mg L(-1) and a limit of quantification (10 sigma) of 0.56 mg L(-1). The relative standard deviations of 1.98% and 1.93% for 5 mg L(-1) and 30 mg L(-1) of the drug, respectively (n = 11) are obtained. The proposed method has been applied satisfactorily to the determination of cefadroxil in commercial pharmaceutical formulations with a sampling rate of 100 h(-1). Results obtained were in good agreement with those obtained by the official HPLC method at the 95% confidence level. 2014-08-30T03:23:49Z 2014-08-30T03:23:49Z 2006 Journal Article 0910-6340 16760604 http://www.ncbi.nlm.nih.gov/pubmed/3502482 http://cmuir.cmu.ac.th/handle/6653943832/6089 eng
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description A sequential injection analysis (SIA) spectrophotometric procedure for cefadroxil determination has been developed. The SIA instrumentation was modified to achieve the desired function and operations by using the software developed to interface the PC with the conventional SIA system. The method is based on the measurement of a red, water-soluble product formed by the reaction between cefadroxil and 4-aminoantipyrine in the presence of alkaline potassium hexacyanoferrate(III) at 510 nm. Optimum conditions for determining the drug were investigated. Beer's law was obeyed over the concentration ranges of 1 - 10 mg L(-1) and 10 - 50 mg L(-1) with a detection limit (3 sigma) of 0.17 mg L(-1) and a limit of quantification (10 sigma) of 0.56 mg L(-1). The relative standard deviations of 1.98% and 1.93% for 5 mg L(-1) and 30 mg L(-1) of the drug, respectively (n = 11) are obtained. The proposed method has been applied satisfactorily to the determination of cefadroxil in commercial pharmaceutical formulations with a sampling rate of 100 h(-1). Results obtained were in good agreement with those obtained by the official HPLC method at the 95% confidence level.
format Article
author Makchit J.
Upalee S.
Thongpoon C.
Liawruangrath B.
Liawruangrath S.
spellingShingle Makchit J.
Upalee S.
Thongpoon C.
Liawruangrath B.
Liawruangrath S.
Determination of cefadroxil by sequential injection with spectrophotometric detector
author_facet Makchit J.
Upalee S.
Thongpoon C.
Liawruangrath B.
Liawruangrath S.
author_sort Makchit J.
title Determination of cefadroxil by sequential injection with spectrophotometric detector
title_short Determination of cefadroxil by sequential injection with spectrophotometric detector
title_full Determination of cefadroxil by sequential injection with spectrophotometric detector
title_fullStr Determination of cefadroxil by sequential injection with spectrophotometric detector
title_full_unstemmed Determination of cefadroxil by sequential injection with spectrophotometric detector
title_sort determination of cefadroxil by sequential injection with spectrophotometric detector
publishDate 2014
url http://www.ncbi.nlm.nih.gov/pubmed/3502482
http://cmuir.cmu.ac.th/handle/6653943832/6089
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