Renoprotective effect of trolox against ischaemia-reperfusion injury in rats

1. Although α-tocopherol has been shown to improve renal function following ischaemia-reperfusion (I/R) injury, its clinical use is not common because α-tocopherol requires several days of pretreatment to exhibit anti-oxidative benefits. The advent of trolox, a water-soluble analogue of α-tocopherol...

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Main Authors: Orawan Wongmekiat, Kamthorn Thamprasert, Dusit Lumlertgul
Format: Journal
Published: 2018
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spelling th-cmuir.6653943832-608942018-09-10T04:09:36Z Renoprotective effect of trolox against ischaemia-reperfusion injury in rats Orawan Wongmekiat Kamthorn Thamprasert Dusit Lumlertgul Biochemistry, Genetics and Molecular Biology Medicine Pharmacology, Toxicology and Pharmaceutics 1. Although α-tocopherol has been shown to improve renal function following ischaemia-reperfusion (I/R) injury, its clinical use is not common because α-tocopherol requires several days of pretreatment to exhibit anti-oxidative benefits. The advent of trolox, a water-soluble analogue of α-tocopherol, has raised the possibility that this compound may function more rapidly during acute oxidative stress than the conventional α-tocopherol. 2. The present study was undertaken to determine the effects of the short-term administration of trolox on renal excretory function following I/R in rats. 3. Male Wistar rats were subjected to 45 min unilateral renal artery occlusion followed by 120 min reperfusion. The control I/R group was subjected to I/R and received saline as an intravenous bolus (2 mL/kg) followed by a continuous infusion of 2 mL/kg per h starting 30 min before ischaemia, whereas the three trolox-treated I/R groups were given an i.v. bolus of trolox (2.5 mg/kg) followed by a continuous infusion (12 mg/kg per h) starting at 30 min before ischaemia, 5 min before reperfusion and 5 min after reperfusion, respectively. Renal function, malondialdehyde, glutathione and histopathology were evaluated. 4. Ischaemia-reperfusion produced a significant deterioration of renal function, which was accompanied by an elevated malondialdehyde and depleted glutathione content. Kidneys from control I/R rats demonstrated tubular cell transformation, brush border loss, vacuolation, cast formation and tubular obstruction. These changes were attenuated by trolox treatment, with the best improvement achieved when trolox was delivered 5 min before reperfusion. 5. The results demonstrate the renoprotective effects of the short-term administration of trolox on I/R injury. These findings indicate the ability of trolox to overcome a major drawback of using α-tocopherol and suggest that trolox may offer a therapeutic advantage over α-tocopherol in acute ischaemic renal failure settings. © 2007 The Authors. 2018-09-10T04:00:56Z 2018-09-10T04:00:56Z 2007-08-01 Journal 14401681 03051870 2-s2.0-34347342682 10.1111/j.1440-1681.2007.04651.x https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=34347342682&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/60894
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
Medicine
Pharmacology, Toxicology and Pharmaceutics
spellingShingle Biochemistry, Genetics and Molecular Biology
Medicine
Pharmacology, Toxicology and Pharmaceutics
Orawan Wongmekiat
Kamthorn Thamprasert
Dusit Lumlertgul
Renoprotective effect of trolox against ischaemia-reperfusion injury in rats
description 1. Although α-tocopherol has been shown to improve renal function following ischaemia-reperfusion (I/R) injury, its clinical use is not common because α-tocopherol requires several days of pretreatment to exhibit anti-oxidative benefits. The advent of trolox, a water-soluble analogue of α-tocopherol, has raised the possibility that this compound may function more rapidly during acute oxidative stress than the conventional α-tocopherol. 2. The present study was undertaken to determine the effects of the short-term administration of trolox on renal excretory function following I/R in rats. 3. Male Wistar rats were subjected to 45 min unilateral renal artery occlusion followed by 120 min reperfusion. The control I/R group was subjected to I/R and received saline as an intravenous bolus (2 mL/kg) followed by a continuous infusion of 2 mL/kg per h starting 30 min before ischaemia, whereas the three trolox-treated I/R groups were given an i.v. bolus of trolox (2.5 mg/kg) followed by a continuous infusion (12 mg/kg per h) starting at 30 min before ischaemia, 5 min before reperfusion and 5 min after reperfusion, respectively. Renal function, malondialdehyde, glutathione and histopathology were evaluated. 4. Ischaemia-reperfusion produced a significant deterioration of renal function, which was accompanied by an elevated malondialdehyde and depleted glutathione content. Kidneys from control I/R rats demonstrated tubular cell transformation, brush border loss, vacuolation, cast formation and tubular obstruction. These changes were attenuated by trolox treatment, with the best improvement achieved when trolox was delivered 5 min before reperfusion. 5. The results demonstrate the renoprotective effects of the short-term administration of trolox on I/R injury. These findings indicate the ability of trolox to overcome a major drawback of using α-tocopherol and suggest that trolox may offer a therapeutic advantage over α-tocopherol in acute ischaemic renal failure settings. © 2007 The Authors.
format Journal
author Orawan Wongmekiat
Kamthorn Thamprasert
Dusit Lumlertgul
author_facet Orawan Wongmekiat
Kamthorn Thamprasert
Dusit Lumlertgul
author_sort Orawan Wongmekiat
title Renoprotective effect of trolox against ischaemia-reperfusion injury in rats
title_short Renoprotective effect of trolox against ischaemia-reperfusion injury in rats
title_full Renoprotective effect of trolox against ischaemia-reperfusion injury in rats
title_fullStr Renoprotective effect of trolox against ischaemia-reperfusion injury in rats
title_full_unstemmed Renoprotective effect of trolox against ischaemia-reperfusion injury in rats
title_sort renoprotective effect of trolox against ischaemia-reperfusion injury in rats
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=34347342682&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/60894
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