Micellar liquid chromatographic determination of penicillins in pharmaceuticals

A new, simple, rapid and specific micellar liquid chromatographic (MLC) method was developed and validated for the determination of amoxicillin, ampicillin, cloxacillin and dicloxacillin in pharmaceutical formulations. Separation was achieved isocratically on an Ultra C18column (150 mm × 4.6 mm) uti...

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Main Authors: P. Srisom, B. Liawruangrath, S. Liawruangrath
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/60902
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-609022018-09-10T04:02:04Z Micellar liquid chromatographic determination of penicillins in pharmaceuticals P. Srisom B. Liawruangrath S. Liawruangrath Biochemistry, Genetics and Molecular Biology Chemistry A new, simple, rapid and specific micellar liquid chromatographic (MLC) method was developed and validated for the determination of amoxicillin, ampicillin, cloxacillin and dicloxacillin in pharmaceutical formulations. Separation was achieved isocratically on an Ultra C18column (150 mm × 4.6 mm) utilizing a mobile phase of 25 mM SDS-2% (v/v) 1-butanol in phosphate buffer (pH 5.0) at a flow rate of 1.0 mL min-1with UV detection at 200 nm. Validation experiments were performed to demonstrate linear ranges, accuracy, precision, robustness, limit of detection (LOD) and limit of quantification (LOQ). The method was applied to the determination of these penicillins in various pharmaceutical formulations. The results compared favorably with those obtained by the official methods, and were in agreement with the declared compositions. The method can be used for quality control assay of the studied penicillins. © 2007 Friedr. Vieweg & Sohn Verlag/GWV Fachverlage GmbH. 2018-09-10T04:01:05Z 2018-09-10T04:01:05Z 2007-06-01 Journal 16121112 00095893 2-s2.0-34250013049 10.1365/s10337-007-0248-0 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=34250013049&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/60902
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
Chemistry
spellingShingle Biochemistry, Genetics and Molecular Biology
Chemistry
P. Srisom
B. Liawruangrath
S. Liawruangrath
Micellar liquid chromatographic determination of penicillins in pharmaceuticals
description A new, simple, rapid and specific micellar liquid chromatographic (MLC) method was developed and validated for the determination of amoxicillin, ampicillin, cloxacillin and dicloxacillin in pharmaceutical formulations. Separation was achieved isocratically on an Ultra C18column (150 mm × 4.6 mm) utilizing a mobile phase of 25 mM SDS-2% (v/v) 1-butanol in phosphate buffer (pH 5.0) at a flow rate of 1.0 mL min-1with UV detection at 200 nm. Validation experiments were performed to demonstrate linear ranges, accuracy, precision, robustness, limit of detection (LOD) and limit of quantification (LOQ). The method was applied to the determination of these penicillins in various pharmaceutical formulations. The results compared favorably with those obtained by the official methods, and were in agreement with the declared compositions. The method can be used for quality control assay of the studied penicillins. © 2007 Friedr. Vieweg & Sohn Verlag/GWV Fachverlage GmbH.
format Journal
author P. Srisom
B. Liawruangrath
S. Liawruangrath
author_facet P. Srisom
B. Liawruangrath
S. Liawruangrath
author_sort P. Srisom
title Micellar liquid chromatographic determination of penicillins in pharmaceuticals
title_short Micellar liquid chromatographic determination of penicillins in pharmaceuticals
title_full Micellar liquid chromatographic determination of penicillins in pharmaceuticals
title_fullStr Micellar liquid chromatographic determination of penicillins in pharmaceuticals
title_full_unstemmed Micellar liquid chromatographic determination of penicillins in pharmaceuticals
title_sort micellar liquid chromatographic determination of penicillins in pharmaceuticals
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=34250013049&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/60902
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