Isolation and characterization of thermotolerant bacterium utilizing ammonium and nitrate ions under aerobic conditions
A thermotolerant bacterium, identified as Bacillus licheniformis, completely utilized 0.1% (w/v) NH4NO3at 30 and 50°C under aerobic condition. The addition of 0.5 mM Fe2+to the NH4NO3medium markedly promoted the utilization of NH4+and NO3-. At 50°C, of total nitrogen originally provided, 24% was tak...
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Main Authors: | , , , , , |
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Format: | Journal |
Published: |
2018
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Subjects: | |
Online Access: | https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33847318870&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/60916 |
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Institution: | Chiang Mai University |
Summary: | A thermotolerant bacterium, identified as Bacillus licheniformis, completely utilized 0.1% (w/v) NH4NO3at 30 and 50°C under aerobic condition. The addition of 0.5 mM Fe2+to the NH4NO3medium markedly promoted the utilization of NH4+and NO3-. At 50°C, of total nitrogen originally provided, 24% was taken up into the cells and 20% remained in the culture supernatant. Residual nitrogen (56%) was probably removed into the atmosphere. The cell extracts contained enzymes involved in denitrification. GC-MS demonstrated that NH415NO3had been converted to15N2O. These results indicate that the strain has denitrification ability under aerobic condition. © 2007 Springer Science+Business Media B.V. |
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