Quercetin, Siamois 1 and Siamois 2 induce apoptosis in human breast cancer MDA-MB-435 cells xenograft in vivo

• Main Authors: Samarn Dechsupa, Suchart Kothan, Jackie Vergote, Gerard Leger, Antoine Martineau, Simone Beranger, Rachain Kosanlavit, Jean Luc Moretti, Samlee Mankhetkorn
• Format: Journal
• Published: 2018
• Subjects: Biochemistry, Genetics and Molecular Biology; Medicine; Pharmacology, Toxicology and Pharmaceutics
• Online Access: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33847123638&origin=inward; http://cmuir.cmu.ac.th/jspui/handle/6653943832/60935
• Institution: Chiang Mai University

We sought to investigate the apoptosis-inducing activities of quercetin, Siamois 1, and Siamois 2 against invasive estrogen-receptor negative MDA-MB 435 cells xenografted in athymic nude mice. This study clearly demonstrated that these compounds exhibited apoptosis-inducing activities in cell culture system. Quercetin (20 μg/mL), Siamois 1 (100 μg/mL), and Siamois 2 (200 μg/mL) can induce apoptotic cell death by 40 ± 5%, 44 ± 14 %, and 31 ± 13 %, respectively. Two-fold of IC50of these compounds were clearly found to induce apoptosis in breast tumor tissue which can be determined by99mTc-Annexin V scintigraphy and histological staining. This is the first report that the apoptosis-inducing effects of quercetin, Siamois 1 and Siamois 2 on the MDA-MB 435 cell in vitro were effectively extrapolated to the in vivo situation. These compounds might be considered as a simple dietary supplement and with further clinical investigation for their use as a nutrition-based intervention in breast cancer treatment. ©2007 Landes Bioscience.