Sequential injection titration with spectrophotometric detection for the assay of acidity in fruit juices

A simple sequential injection analysis (SIA) with spectrophotometric detection for an assay of acidity in fruit juice was investigated. An alkaline reagent (sodium hydroxide), a sample and an indicator (phenolphthalein) were first aspirated and stacked as adjacent zones in a holding coil. With flow...

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Main Authors: Jakmunee J., Rujiralai T., Grudpan K.
Format: Article
Language:English
Published: 2014
Online Access:http://www.ncbi.nlm.nih.gov/pubmed/3502482
http://cmuir.cmu.ac.th/handle/6653943832/6097
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Institution: Chiang Mai University
Language: English
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spelling th-cmuir.6653943832-60972014-08-30T03:23:50Z Sequential injection titration with spectrophotometric detection for the assay of acidity in fruit juices Jakmunee J. Rujiralai T. Grudpan K. A simple sequential injection analysis (SIA) with spectrophotometric detection for an assay of acidity in fruit juice was investigated. An alkaline reagent (sodium hydroxide), a sample and an indicator (phenolphthalein) were first aspirated and stacked as adjacent zones in a holding coil. With flow reversal through a reaction coil to the detector, zone penetration occurred, leading to a neutralization reaction that caused a decrease in the color intensity of the indicator being monitored for absorbance at 552 nm. The effects of various parameters were studied. Linear calibration graphs for acidities of 0.2 - 1.0 and 0.5 - 2.5% w/v citric acid as a standard, with a relative standard deviation of 1% (acidity of 0.3 - 0.6% w/v as citric acid, n=11) and a sample throughput of 30 samples h(-1), were achieved. The developed method was validated by a standard titrimetric method for assaying the acidity of fruit juice samples. 2014-08-30T03:23:50Z 2014-08-30T03:23:50Z 2006 Journal Article 0910-6340 16429794 http://www.ncbi.nlm.nih.gov/pubmed/3502482 http://cmuir.cmu.ac.th/handle/6653943832/6097 eng
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description A simple sequential injection analysis (SIA) with spectrophotometric detection for an assay of acidity in fruit juice was investigated. An alkaline reagent (sodium hydroxide), a sample and an indicator (phenolphthalein) were first aspirated and stacked as adjacent zones in a holding coil. With flow reversal through a reaction coil to the detector, zone penetration occurred, leading to a neutralization reaction that caused a decrease in the color intensity of the indicator being monitored for absorbance at 552 nm. The effects of various parameters were studied. Linear calibration graphs for acidities of 0.2 - 1.0 and 0.5 - 2.5% w/v citric acid as a standard, with a relative standard deviation of 1% (acidity of 0.3 - 0.6% w/v as citric acid, n=11) and a sample throughput of 30 samples h(-1), were achieved. The developed method was validated by a standard titrimetric method for assaying the acidity of fruit juice samples.
format Article
author Jakmunee J.
Rujiralai T.
Grudpan K.
spellingShingle Jakmunee J.
Rujiralai T.
Grudpan K.
Sequential injection titration with spectrophotometric detection for the assay of acidity in fruit juices
author_facet Jakmunee J.
Rujiralai T.
Grudpan K.
author_sort Jakmunee J.
title Sequential injection titration with spectrophotometric detection for the assay of acidity in fruit juices
title_short Sequential injection titration with spectrophotometric detection for the assay of acidity in fruit juices
title_full Sequential injection titration with spectrophotometric detection for the assay of acidity in fruit juices
title_fullStr Sequential injection titration with spectrophotometric detection for the assay of acidity in fruit juices
title_full_unstemmed Sequential injection titration with spectrophotometric detection for the assay of acidity in fruit juices
title_sort sequential injection titration with spectrophotometric detection for the assay of acidity in fruit juices
publishDate 2014
url http://www.ncbi.nlm.nih.gov/pubmed/3502482
http://cmuir.cmu.ac.th/handle/6653943832/6097
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