Sequential injection titration with spectrophotometric detection for the assay of acidity in fruit juices
A simple sequential injection analysis (SIA) with spectrophotometric detection for an assay of acidity in fruit juice was investigated. An alkaline reagent (sodium hydroxide), a sample and an indicator (phenolphthalein) were first aspirated and stacked as adjacent zones in a holding coil. With flow...
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th-cmuir.6653943832-60972014-08-30T03:23:50Z Sequential injection titration with spectrophotometric detection for the assay of acidity in fruit juices Jakmunee J. Rujiralai T. Grudpan K. A simple sequential injection analysis (SIA) with spectrophotometric detection for an assay of acidity in fruit juice was investigated. An alkaline reagent (sodium hydroxide), a sample and an indicator (phenolphthalein) were first aspirated and stacked as adjacent zones in a holding coil. With flow reversal through a reaction coil to the detector, zone penetration occurred, leading to a neutralization reaction that caused a decrease in the color intensity of the indicator being monitored for absorbance at 552 nm. The effects of various parameters were studied. Linear calibration graphs for acidities of 0.2 - 1.0 and 0.5 - 2.5% w/v citric acid as a standard, with a relative standard deviation of 1% (acidity of 0.3 - 0.6% w/v as citric acid, n=11) and a sample throughput of 30 samples h(-1), were achieved. The developed method was validated by a standard titrimetric method for assaying the acidity of fruit juice samples. 2014-08-30T03:23:50Z 2014-08-30T03:23:50Z 2006 Journal Article 0910-6340 16429794 http://www.ncbi.nlm.nih.gov/pubmed/3502482 http://cmuir.cmu.ac.th/handle/6653943832/6097 eng |
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A simple sequential injection analysis (SIA) with spectrophotometric detection for an assay of acidity in fruit juice was investigated. An alkaline reagent (sodium hydroxide), a sample and an indicator (phenolphthalein) were first aspirated and stacked as adjacent zones in a holding coil. With flow reversal through a reaction coil to the detector, zone penetration occurred, leading to a neutralization reaction that caused a decrease in the color intensity of the indicator being monitored for absorbance at 552 nm. The effects of various parameters were studied. Linear calibration graphs for acidities of 0.2 - 1.0 and 0.5 - 2.5% w/v citric acid as a standard, with a relative standard deviation of 1% (acidity of 0.3 - 0.6% w/v as citric acid, n=11) and a sample throughput of 30 samples h(-1), were achieved. The developed method was validated by a standard titrimetric method for assaying the acidity of fruit juice samples. |
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Article |
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Jakmunee J. Rujiralai T. Grudpan K. |
spellingShingle |
Jakmunee J. Rujiralai T. Grudpan K. Sequential injection titration with spectrophotometric detection for the assay of acidity in fruit juices |
author_facet |
Jakmunee J. Rujiralai T. Grudpan K. |
author_sort |
Jakmunee J. |
title |
Sequential injection titration with spectrophotometric detection for the assay of acidity in fruit juices |
title_short |
Sequential injection titration with spectrophotometric detection for the assay of acidity in fruit juices |
title_full |
Sequential injection titration with spectrophotometric detection for the assay of acidity in fruit juices |
title_fullStr |
Sequential injection titration with spectrophotometric detection for the assay of acidity in fruit juices |
title_full_unstemmed |
Sequential injection titration with spectrophotometric detection for the assay of acidity in fruit juices |
title_sort |
sequential injection titration with spectrophotometric detection for the assay of acidity in fruit juices |
publishDate |
2014 |
url |
http://www.ncbi.nlm.nih.gov/pubmed/3502482 http://cmuir.cmu.ac.th/handle/6653943832/6097 |
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1681420549680529408 |