Molecular characterization of rare G3P[9] rotavirus strains isolated from children hospitalized with acute gastroenteritis

In 2004, an epidemiological survey of human rotavirus infection in Chiang Mai, Thailand detected two uncommon human rotavirus strains (CMH120/04 and CMH134/04) bearing AU-1-like G3P[9] genotypes in 1 year old children hospitalized with acute gastroenteritis. The CMH120/04 and CMH134/04 rotavirus str...

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Main Authors: Pattara Khamrin, Niwat Maneekarn, Supatra Peerakome, Supin Tonusin, Gia Phan Tung, Shoko Okitsu, Hiroshi Ushijima
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/61134
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spelling th-cmuir.6653943832-611342018-09-10T04:08:14Z Molecular characterization of rare G3P[9] rotavirus strains isolated from children hospitalized with acute gastroenteritis Pattara Khamrin Niwat Maneekarn Supatra Peerakome Supin Tonusin Gia Phan Tung Shoko Okitsu Hiroshi Ushijima Immunology and Microbiology Medicine In 2004, an epidemiological survey of human rotavirus infection in Chiang Mai, Thailand detected two uncommon human rotavirus strains (CMH120/04 and CMH134/04) bearing AU-1-like G3P[9] genotypes in 1 year old children hospitalized with acute gastroenteritis. The CMH120/04 and CMH134/04 rotavirus strains were characterized by molecular analyses of their VP6, VP7, VP8*, and NSP4 gene segments as well as the determination of RNA patterns by polyacrylamide gel electrophoresis (PAGE). Analysis of the VP8* gene revealed a high level of amino acid sequence identities with those of P[9] rotavirus reference strains, ranging from 94.9% to 98.3%. The highest identities were shared with the human rotavirus AU-1 strain at 97.8% and 98.3% for CMH120/04 and CMH134/04 strains, respectively. Analysis of the VP7 gene sequence revealed the highest identities with G3 human rotavirus strain KC814 at 96.6% and 96.2% for CMH120/04 and CMH134/ 04 strains, respectively. Based on the analyses of VP7 and VP8* genes, CMH120/04 and CMH134/04 belonged to G3P[9] genotypes. In addition, analyses of VP6 and NSP4 sequences revealed a VP6 subgroup (SG) I, with NSP4 genetic group C specificities. Moreover, both strains displayed a long RNA electrophoretic pattern. The finding of uncommon G3P[9] rotaviruses in pediatric patients provided additional evidence of the genetic/antigenic diversities of human group A rotaviruses in the Chiang Mai area of Thailand. © 2007 Wiley-Liss, Inc. 2018-09-10T04:05:16Z 2018-09-10T04:05:16Z 2007-06-01 Journal 10969071 01466615 2-s2.0-34248167778 10.1002/jmv.20840 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=34248167778&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/61134
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Immunology and Microbiology
Medicine
spellingShingle Immunology and Microbiology
Medicine
Pattara Khamrin
Niwat Maneekarn
Supatra Peerakome
Supin Tonusin
Gia Phan Tung
Shoko Okitsu
Hiroshi Ushijima
Molecular characterization of rare G3P[9] rotavirus strains isolated from children hospitalized with acute gastroenteritis
description In 2004, an epidemiological survey of human rotavirus infection in Chiang Mai, Thailand detected two uncommon human rotavirus strains (CMH120/04 and CMH134/04) bearing AU-1-like G3P[9] genotypes in 1 year old children hospitalized with acute gastroenteritis. The CMH120/04 and CMH134/04 rotavirus strains were characterized by molecular analyses of their VP6, VP7, VP8*, and NSP4 gene segments as well as the determination of RNA patterns by polyacrylamide gel electrophoresis (PAGE). Analysis of the VP8* gene revealed a high level of amino acid sequence identities with those of P[9] rotavirus reference strains, ranging from 94.9% to 98.3%. The highest identities were shared with the human rotavirus AU-1 strain at 97.8% and 98.3% for CMH120/04 and CMH134/04 strains, respectively. Analysis of the VP7 gene sequence revealed the highest identities with G3 human rotavirus strain KC814 at 96.6% and 96.2% for CMH120/04 and CMH134/ 04 strains, respectively. Based on the analyses of VP7 and VP8* genes, CMH120/04 and CMH134/04 belonged to G3P[9] genotypes. In addition, analyses of VP6 and NSP4 sequences revealed a VP6 subgroup (SG) I, with NSP4 genetic group C specificities. Moreover, both strains displayed a long RNA electrophoretic pattern. The finding of uncommon G3P[9] rotaviruses in pediatric patients provided additional evidence of the genetic/antigenic diversities of human group A rotaviruses in the Chiang Mai area of Thailand. © 2007 Wiley-Liss, Inc.
format Journal
author Pattara Khamrin
Niwat Maneekarn
Supatra Peerakome
Supin Tonusin
Gia Phan Tung
Shoko Okitsu
Hiroshi Ushijima
author_facet Pattara Khamrin
Niwat Maneekarn
Supatra Peerakome
Supin Tonusin
Gia Phan Tung
Shoko Okitsu
Hiroshi Ushijima
author_sort Pattara Khamrin
title Molecular characterization of rare G3P[9] rotavirus strains isolated from children hospitalized with acute gastroenteritis
title_short Molecular characterization of rare G3P[9] rotavirus strains isolated from children hospitalized with acute gastroenteritis
title_full Molecular characterization of rare G3P[9] rotavirus strains isolated from children hospitalized with acute gastroenteritis
title_fullStr Molecular characterization of rare G3P[9] rotavirus strains isolated from children hospitalized with acute gastroenteritis
title_full_unstemmed Molecular characterization of rare G3P[9] rotavirus strains isolated from children hospitalized with acute gastroenteritis
title_sort molecular characterization of rare g3p[9] rotavirus strains isolated from children hospitalized with acute gastroenteritis
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=34248167778&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/61134
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