Intracellular growth and drug susceptibility of Mycobacterium tuberculosis in macrophages

Intracellular growth of Mycobacterium tuberculosis in human and rabbit monocytes and in mouse and guinea pig macrophages was evaluated. Monocytes or macrophages were infected with M. tuberculosis H37Rv with the multiplicity of infection at 10 mycobacteria per monocyte. The average percentages of inf...

Full description

Saved in:
Bibliographic Details
Main Authors: Sakarin Chanwong, Niwat Maneekarn, Luksana Makonkawkeyoon, Sanit Makonkawkeyoon
Format: Journal
Published: 2018
Subjects:
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33846814925&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/61143
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Chiang Mai University
id th-cmuir.6653943832-61143
record_format dspace
spelling th-cmuir.6653943832-611432018-09-10T04:11:28Z Intracellular growth and drug susceptibility of Mycobacterium tuberculosis in macrophages Sakarin Chanwong Niwat Maneekarn Luksana Makonkawkeyoon Sanit Makonkawkeyoon Immunology and Microbiology Medicine Veterinary Intracellular growth of Mycobacterium tuberculosis in human and rabbit monocytes and in mouse and guinea pig macrophages was evaluated. Monocytes or macrophages were infected with M. tuberculosis H37Rv with the multiplicity of infection at 10 mycobacteria per monocyte. The average percentages of infected human and rabbit monocytes were 22% and 19%, while mouse and guinea pig macrophages were 46% and 58%, respectively. The average generation times of M. tuberculosis H37Rv inside human and rabbit monocytes and in mouse and guinea pig macrophages, after culturing the infected cells for 10 days, were 33.4, 50.3, 31.4, and 25.6 h, respectively. Using infected guinea pig macrophages for intracellular evaluation of drug susceptibility, the minimal inhibitory concentrations (MICs) of isoniazid to the intracellular and extracellular M. tuberculosis H37Rv were 0.1 and 0.4 μg/ml, while the MICs of rifampicin were 0.1 and 0.2 μg/ml, respectively. The minimal bactericidal concentrations (MBCs) of isoniazid to the intracellular and extracellular H37Rv were 0.2 and 0.4 μg/ml, while the MCSs of rifampicin were 0.1 and 0.2 μg/ml, respectively. © 2006 Elsevier Ltd. All rights reserved. 2018-09-10T04:05:28Z 2018-09-10T04:05:28Z 2007-03-01 Journal 14729792 2-s2.0-33846814925 10.1016/j.tube.2006.06.001 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33846814925&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/61143
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Immunology and Microbiology
Medicine
Veterinary
spellingShingle Immunology and Microbiology
Medicine
Veterinary
Sakarin Chanwong
Niwat Maneekarn
Luksana Makonkawkeyoon
Sanit Makonkawkeyoon
Intracellular growth and drug susceptibility of Mycobacterium tuberculosis in macrophages
description Intracellular growth of Mycobacterium tuberculosis in human and rabbit monocytes and in mouse and guinea pig macrophages was evaluated. Monocytes or macrophages were infected with M. tuberculosis H37Rv with the multiplicity of infection at 10 mycobacteria per monocyte. The average percentages of infected human and rabbit monocytes were 22% and 19%, while mouse and guinea pig macrophages were 46% and 58%, respectively. The average generation times of M. tuberculosis H37Rv inside human and rabbit monocytes and in mouse and guinea pig macrophages, after culturing the infected cells for 10 days, were 33.4, 50.3, 31.4, and 25.6 h, respectively. Using infected guinea pig macrophages for intracellular evaluation of drug susceptibility, the minimal inhibitory concentrations (MICs) of isoniazid to the intracellular and extracellular M. tuberculosis H37Rv were 0.1 and 0.4 μg/ml, while the MICs of rifampicin were 0.1 and 0.2 μg/ml, respectively. The minimal bactericidal concentrations (MBCs) of isoniazid to the intracellular and extracellular H37Rv were 0.2 and 0.4 μg/ml, while the MCSs of rifampicin were 0.1 and 0.2 μg/ml, respectively. © 2006 Elsevier Ltd. All rights reserved.
format Journal
author Sakarin Chanwong
Niwat Maneekarn
Luksana Makonkawkeyoon
Sanit Makonkawkeyoon
author_facet Sakarin Chanwong
Niwat Maneekarn
Luksana Makonkawkeyoon
Sanit Makonkawkeyoon
author_sort Sakarin Chanwong
title Intracellular growth and drug susceptibility of Mycobacterium tuberculosis in macrophages
title_short Intracellular growth and drug susceptibility of Mycobacterium tuberculosis in macrophages
title_full Intracellular growth and drug susceptibility of Mycobacterium tuberculosis in macrophages
title_fullStr Intracellular growth and drug susceptibility of Mycobacterium tuberculosis in macrophages
title_full_unstemmed Intracellular growth and drug susceptibility of Mycobacterium tuberculosis in macrophages
title_sort intracellular growth and drug susceptibility of mycobacterium tuberculosis in macrophages
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33846814925&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/61143
_version_ 1681425565906632704