High annealing temperature-random amplified polymorphic DNA (HAT-RAPD) analysis of three paramphistome flukes from Thailand

High annealing temperature-random amplified polymorphic DNA (HAT-RAPD) analysis of three paramphistome flukes from Thailand

The rumen flukes of 37 cows (Bos indicus) from Chiang Mai and Lamphun provinces were investigated, and the overall prevalence of infection was 78.38% (29/37). Three species were found: Paramphistomum epiclitum, Orthocoelium streptocoelium, and Fischoederius elongatus with prevalences of infection of...

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Main Authors: Pralongyut Sripalwit, Chalobol Wongsawad, Pheravut Wongsawad, Somboon Anuntalabhochai
Format: Journal
Published: 2018
Subjects:
Immunology and Microbiology
Medicine
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33751186329&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/61148
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-611482018-09-10T04:09:04Z High annealing temperature-random amplified polymorphic DNA (HAT-RAPD) analysis of three paramphistome flukes from Thailand Pralongyut Sripalwit Chalobol Wongsawad Pheravut Wongsawad Somboon Anuntalabhochai Immunology and Microbiology Medicine The rumen flukes of 37 cows (Bos indicus) from Chiang Mai and Lamphun provinces were investigated, and the overall prevalence of infection was 78.38% (29/37). Three species were found: Paramphistomum epiclitum, Orthocoelium streptocoelium, and Fischoederius elongatus with prevalences of infection of 75.68%, 48.65%, and 40.54%, respectively. Genomic DNA was amplified by polymerase chain reaction based on the high annealing temperature-random amplification of polymorphic DNA (HAT-RAPD) technique. Five random 10-mer oligonucleotide primers (OPA2, OPA4, OPB18, OPC9, and OPH11) produced distinct banding patterns in three species. No genetic variations in these three species were identified using 10 arbitary primers. © 2006 Elsevier Inc. All rights reserved. 2018-09-10T04:05:32Z 2018-09-10T04:05:32Z 2007-01-01 Journal 10902449 00144894 2-s2.0-33751186329 10.1016/j.exppara.2006.05.005 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33751186329&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/61148
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Immunology and Microbiology
Medicine
spellingShingle Immunology and Microbiology
Medicine
Pralongyut Sripalwit
Chalobol Wongsawad
Pheravut Wongsawad
Somboon Anuntalabhochai
High annealing temperature-random amplified polymorphic DNA (HAT-RAPD) analysis of three paramphistome flukes from Thailand
description The rumen flukes of 37 cows (Bos indicus) from Chiang Mai and Lamphun provinces were investigated, and the overall prevalence of infection was 78.38% (29/37). Three species were found: Paramphistomum epiclitum, Orthocoelium streptocoelium, and Fischoederius elongatus with prevalences of infection of 75.68%, 48.65%, and 40.54%, respectively. Genomic DNA was amplified by polymerase chain reaction based on the high annealing temperature-random amplification of polymorphic DNA (HAT-RAPD) technique. Five random 10-mer oligonucleotide primers (OPA2, OPA4, OPB18, OPC9, and OPH11) produced distinct banding patterns in three species. No genetic variations in these three species were identified using 10 arbitary primers. © 2006 Elsevier Inc. All rights reserved.
format Journal
author Pralongyut Sripalwit
Chalobol Wongsawad
Pheravut Wongsawad
Somboon Anuntalabhochai
author_facet Pralongyut Sripalwit
Chalobol Wongsawad
Pheravut Wongsawad
Somboon Anuntalabhochai
author_sort Pralongyut Sripalwit
title High annealing temperature-random amplified polymorphic DNA (HAT-RAPD) analysis of three paramphistome flukes from Thailand
title_short High annealing temperature-random amplified polymorphic DNA (HAT-RAPD) analysis of three paramphistome flukes from Thailand
title_full High annealing temperature-random amplified polymorphic DNA (HAT-RAPD) analysis of three paramphistome flukes from Thailand
title_fullStr High annealing temperature-random amplified polymorphic DNA (HAT-RAPD) analysis of three paramphistome flukes from Thailand
title_full_unstemmed High annealing temperature-random amplified polymorphic DNA (HAT-RAPD) analysis of three paramphistome flukes from Thailand
title_sort high annealing temperature-random amplified polymorphic dna (hat-rapd) analysis of three paramphistome flukes from thailand
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33751186329&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/61148
_version_ 1681425566814699520

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