Random amplified polymorphic DNA typing and phylogeny of Pythium insidiosum clinical isolates in Thailand

Forty-three Pythium insidiosum clinical isolates recovered from human pythiosis cases in Thailand were characterized by random amplified polymorphic DNA (RAPD) analysis. Three random oligonucleotide primers, OPW11, OPW12 and OPX13 generated 39, 34 and 35 DNA patterns with high value of typeability (...

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Main Authors: Srisuda Pannanusorn, Angkana Chaiprasert, Chularut Prariyachatigul, Theerapong Krajaejun, Nongnuch Vanittanakom, Ariya Chindamporn, Wanchai Wanachiwanawin, Boonmee Satapatayavong
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Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/61336
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-613362018-09-10T04:08:48Z Random amplified polymorphic DNA typing and phylogeny of Pythium insidiosum clinical isolates in Thailand Srisuda Pannanusorn Angkana Chaiprasert Chularut Prariyachatigul Theerapong Krajaejun Nongnuch Vanittanakom Ariya Chindamporn Wanchai Wanachiwanawin Boonmee Satapatayavong Medicine Forty-three Pythium insidiosum clinical isolates recovered from human pythiosis cases in Thailand were characterized by random amplified polymorphic DNA (RAPD) analysis. Three random oligonucleotide primers, OPW11, OPW12 and OPX13 generated 39, 34 and 35 DNA patterns with high value of typeability (100%), reproducibility (98.5, 88.8 and 93.3%) and discriminatory power (0.83, 0.82 and 0.77), respectively. Using GelCompar software based on band similarity, the 43 clinical isolates of P. insidiosum could be arranged into 9, 13 and 11 clades using OPW11, OPW12 and OPX13, respectively and the combination of all three primers revealed 36 RAPD patterns. Members in each RAPD pattern varied in both clinical forms and/or geographical locations. RAPD pattern 15 was found in 6 isolates, half of which were found in central region of Thailand. Isolates MCC15 and MCC16 isolated from different patients exhibited Identical pattern with all three primers. Our results revealed high genetic heterogeneity among Pythium insidiosum isolates in Thailand. RAPD method should be appropriate for future epidemiological studies of P. insidiosum strains from patients and from natural habitats. 2018-09-10T04:08:48Z 2018-09-10T04:08:48Z 2007-03-01 Journal 01251562 2-s2.0-34249667824 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=34249667824&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/61336
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Medicine
spellingShingle Medicine
Srisuda Pannanusorn
Angkana Chaiprasert
Chularut Prariyachatigul
Theerapong Krajaejun
Nongnuch Vanittanakom
Ariya Chindamporn
Wanchai Wanachiwanawin
Boonmee Satapatayavong
Random amplified polymorphic DNA typing and phylogeny of Pythium insidiosum clinical isolates in Thailand
description Forty-three Pythium insidiosum clinical isolates recovered from human pythiosis cases in Thailand were characterized by random amplified polymorphic DNA (RAPD) analysis. Three random oligonucleotide primers, OPW11, OPW12 and OPX13 generated 39, 34 and 35 DNA patterns with high value of typeability (100%), reproducibility (98.5, 88.8 and 93.3%) and discriminatory power (0.83, 0.82 and 0.77), respectively. Using GelCompar software based on band similarity, the 43 clinical isolates of P. insidiosum could be arranged into 9, 13 and 11 clades using OPW11, OPW12 and OPX13, respectively and the combination of all three primers revealed 36 RAPD patterns. Members in each RAPD pattern varied in both clinical forms and/or geographical locations. RAPD pattern 15 was found in 6 isolates, half of which were found in central region of Thailand. Isolates MCC15 and MCC16 isolated from different patients exhibited Identical pattern with all three primers. Our results revealed high genetic heterogeneity among Pythium insidiosum isolates in Thailand. RAPD method should be appropriate for future epidemiological studies of P. insidiosum strains from patients and from natural habitats.
format Journal
author Srisuda Pannanusorn
Angkana Chaiprasert
Chularut Prariyachatigul
Theerapong Krajaejun
Nongnuch Vanittanakom
Ariya Chindamporn
Wanchai Wanachiwanawin
Boonmee Satapatayavong
author_facet Srisuda Pannanusorn
Angkana Chaiprasert
Chularut Prariyachatigul
Theerapong Krajaejun
Nongnuch Vanittanakom
Ariya Chindamporn
Wanchai Wanachiwanawin
Boonmee Satapatayavong
author_sort Srisuda Pannanusorn
title Random amplified polymorphic DNA typing and phylogeny of Pythium insidiosum clinical isolates in Thailand
title_short Random amplified polymorphic DNA typing and phylogeny of Pythium insidiosum clinical isolates in Thailand
title_full Random amplified polymorphic DNA typing and phylogeny of Pythium insidiosum clinical isolates in Thailand
title_fullStr Random amplified polymorphic DNA typing and phylogeny of Pythium insidiosum clinical isolates in Thailand
title_full_unstemmed Random amplified polymorphic DNA typing and phylogeny of Pythium insidiosum clinical isolates in Thailand
title_sort random amplified polymorphic dna typing and phylogeny of pythium insidiosum clinical isolates in thailand
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=34249667824&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/61336
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