High-performance liquid chromatographic determination of arbutin in skin-whitening creams and medicinal plant extracts

A high-performance liquid chromatographic method was developed for quantitative analysis of arbutin. The arbutin was separated on an ODS Hypersil® C18column with a mobile phase of water:methanol:0.1 M hydrochloric acid (89:10:1, v/v/v). The level of arbutin was measured by means of UV detection at 2...

Full description

Saved in:
Bibliographic Details
Main Authors: Wisanu Thongchai, Boonsom Liawruangrath, Saisunee Liawruangrath
Format: Journal
Published: 2018
Subjects:
Online Access:https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=34147200627&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/61349
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Chiang Mai University
id th-cmuir.6653943832-61349
record_format dspace
spelling th-cmuir.6653943832-613492018-09-10T04:08:57Z High-performance liquid chromatographic determination of arbutin in skin-whitening creams and medicinal plant extracts Wisanu Thongchai Boonsom Liawruangrath Saisunee Liawruangrath Medicine A high-performance liquid chromatographic method was developed for quantitative analysis of arbutin. The arbutin was separated on an ODS Hypersil® C18column with a mobile phase of water:methanol:0.1 M hydrochloric acid (89:10:1, v/v/v). The level of arbutin was measured by means of UV detection at 222 nm. The optimum conditions for arbutin quantitative analysis were investigated. The calibration curve was found to be linear up to 1,000 μg/ml-1of arbutin concentration, and the working calibration curve for arbutin determination over the range 0.5-30.0 μg/ml-1of arbutin (r2= 0.9999) was established. The relative standard deviations for intraday and interday were found to be 0.98% and 1.15%, respectively. A detection limit (3σ) and quantitation limit (10σ) of 0.02 μg/ml-1and 0.2 μg/ml-1, respectively, and a mean percentage recovery of the spiked arbutin of 99.88 ± 1.12% were obtained. The proposed method has been applied to the determination of arbutin in commercial skin-whitening creams (Arbuwhite® cream, Super Whitening® cream, and Shiseido® cream) with average contents of 7.60, 5.30, and 57.90 mg/g-1, respectively. It was also applied to the determination of arbutin in medicinal plant extracts from Betula alnoides Buch. Ham., Clerodendrum petasites S. Moore, Curculigo latifolia Dryand. Var. latifolia, and Hesperetbusa crenulata (Roxb.) Roem, levels of which were found to be 3.50, 1.50, 1.10, and 0.12 μg/g-1, respectively (no article reported in the literature about arbutin analysis). The proposed HPLC method is rapid, simple, and selective for routine analysis. 2018-09-10T04:08:57Z 2018-09-10T04:08:57Z 2007-01-01 Journal 15257886 2-s2.0-34147200627 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=34147200627&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/61349
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Medicine
spellingShingle Medicine
Wisanu Thongchai
Boonsom Liawruangrath
Saisunee Liawruangrath
High-performance liquid chromatographic determination of arbutin in skin-whitening creams and medicinal plant extracts
description A high-performance liquid chromatographic method was developed for quantitative analysis of arbutin. The arbutin was separated on an ODS Hypersil® C18column with a mobile phase of water:methanol:0.1 M hydrochloric acid (89:10:1, v/v/v). The level of arbutin was measured by means of UV detection at 222 nm. The optimum conditions for arbutin quantitative analysis were investigated. The calibration curve was found to be linear up to 1,000 μg/ml-1of arbutin concentration, and the working calibration curve for arbutin determination over the range 0.5-30.0 μg/ml-1of arbutin (r2= 0.9999) was established. The relative standard deviations for intraday and interday were found to be 0.98% and 1.15%, respectively. A detection limit (3σ) and quantitation limit (10σ) of 0.02 μg/ml-1and 0.2 μg/ml-1, respectively, and a mean percentage recovery of the spiked arbutin of 99.88 ± 1.12% were obtained. The proposed method has been applied to the determination of arbutin in commercial skin-whitening creams (Arbuwhite® cream, Super Whitening® cream, and Shiseido® cream) with average contents of 7.60, 5.30, and 57.90 mg/g-1, respectively. It was also applied to the determination of arbutin in medicinal plant extracts from Betula alnoides Buch. Ham., Clerodendrum petasites S. Moore, Curculigo latifolia Dryand. Var. latifolia, and Hesperetbusa crenulata (Roxb.) Roem, levels of which were found to be 3.50, 1.50, 1.10, and 0.12 μg/g-1, respectively (no article reported in the literature about arbutin analysis). The proposed HPLC method is rapid, simple, and selective for routine analysis.
format Journal
author Wisanu Thongchai
Boonsom Liawruangrath
Saisunee Liawruangrath
author_facet Wisanu Thongchai
Boonsom Liawruangrath
Saisunee Liawruangrath
author_sort Wisanu Thongchai
title High-performance liquid chromatographic determination of arbutin in skin-whitening creams and medicinal plant extracts
title_short High-performance liquid chromatographic determination of arbutin in skin-whitening creams and medicinal plant extracts
title_full High-performance liquid chromatographic determination of arbutin in skin-whitening creams and medicinal plant extracts
title_fullStr High-performance liquid chromatographic determination of arbutin in skin-whitening creams and medicinal plant extracts
title_full_unstemmed High-performance liquid chromatographic determination of arbutin in skin-whitening creams and medicinal plant extracts
title_sort high-performance liquid chromatographic determination of arbutin in skin-whitening creams and medicinal plant extracts
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=34147200627&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/61349
_version_ 1681425604164976640