$Purification of papain from Carica papaya latex: Aqueous two-phase extraction versus two-step salt precipitation$

Purification of papain from Carica papaya latex: Aqueous two-phase extraction versus two-step salt precipitation

Purification of papain from wet Carica papaya latex by extraction in aqueous two-phase system was studied and compared with the traditional procedure involving a two-step salt precipitation. The papain obtained by the latter method was usually contaminated with other proteins, and its purity was dep...

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Main Authors:	Sarote Nitsawang, Rajni Hatti-Kaul, Pawinee Kanasawud
Format:	Journal
Published:	2018
Subjects:	Biochemistry, Genetics and Molecular Biology Immunology and Microbiology
Online Access:	https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33745959045&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/61506
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Institution:	Chiang Mai University

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spelling	th-cmuir.6653943832-615062018-09-11T08:57:41Z Purification of papain from Carica papaya latex: Aqueous two-phase extraction versus two-step salt precipitation Sarote Nitsawang Rajni Hatti-Kaul Pawinee Kanasawud Biochemistry, Genetics and Molecular Biology Immunology and Microbiology Purification of papain from wet Carica papaya latex by extraction in aqueous two-phase system was studied and compared with the traditional procedure involving a two-step salt precipitation. The papain obtained by the latter method was usually contaminated with other proteins, and its purity was dependent on the initial protein concentration in the material used for processing. Highly pure papain was obtained in a much shorter processing time directly from unclarified latex with the use of an aqueous two-phase system consisting of 8% (w/w) polyethylene glycol and 15% (w/w) ammonium sulfate. © 2006 Elsevier Inc. All rights reserved. 2018-09-11T08:54:19Z 2018-09-11T08:54:19Z 2006-09-04 Journal 01410229 2-s2.0-33745959045 10.1016/j.enzmictec.2006.02.013 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33745959045&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/61506
institution	Chiang Mai University
building	Chiang Mai University Library
country	Thailand
collection	CMU Intellectual Repository
topic	Biochemistry, Genetics and Molecular Biology Immunology and Microbiology
spellingShingle	Biochemistry, Genetics and Molecular Biology Immunology and Microbiology Sarote Nitsawang Rajni Hatti-Kaul Pawinee Kanasawud Purification of papain from Carica papaya latex: Aqueous two-phase extraction versus two-step salt precipitation
description	Purification of papain from wet Carica papaya latex by extraction in aqueous two-phase system was studied and compared with the traditional procedure involving a two-step salt precipitation. The papain obtained by the latter method was usually contaminated with other proteins, and its purity was dependent on the initial protein concentration in the material used for processing. Highly pure papain was obtained in a much shorter processing time directly from unclarified latex with the use of an aqueous two-phase system consisting of 8% (w/w) polyethylene glycol and 15% (w/w) ammonium sulfate. © 2006 Elsevier Inc. All rights reserved.
format	Journal
author	Sarote Nitsawang Rajni Hatti-Kaul Pawinee Kanasawud
author_facet	Sarote Nitsawang Rajni Hatti-Kaul Pawinee Kanasawud
author_sort	Sarote Nitsawang
title	Purification of papain from Carica papaya latex: Aqueous two-phase extraction versus two-step salt precipitation
title_short	Purification of papain from Carica papaya latex: Aqueous two-phase extraction versus two-step salt precipitation
title_full	Purification of papain from Carica papaya latex: Aqueous two-phase extraction versus two-step salt precipitation
title_fullStr	Purification of papain from Carica papaya latex: Aqueous two-phase extraction versus two-step salt precipitation
title_full_unstemmed	Purification of papain from Carica papaya latex: Aqueous two-phase extraction versus two-step salt precipitation
title_sort	purification of papain from carica papaya latex: aqueous two-phase extraction versus two-step salt precipitation
publishDate	2018
url	https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33745959045&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/61506
_version_	1681425633511473152

Purification of papain from Carica papaya latex: Aqueous two-phase extraction versus two-step salt precipitation

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