An absorbance-based micro-fluidic sensor for diffusion coefficient and molar mass determinations

The H-Sensor reported herein is a micro-fluidic device compatible with flow injection analysis (FIA) and high performance liquid chromatography (HPLC). The device detects analytes at two separate off-chip absorbance flow cells, providing two simultaneous absorbance measurements. The ratio of these t...

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Main Authors: Adam D. McBrady, Rattikan Chantiwas, Ana Kristine Torgerson, Kate Grudpan, Robert E. Synovec
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/61511
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-615112018-09-11T08:57:14Z An absorbance-based micro-fluidic sensor for diffusion coefficient and molar mass determinations Adam D. McBrady Rattikan Chantiwas Ana Kristine Torgerson Kate Grudpan Robert E. Synovec Biochemistry, Genetics and Molecular Biology Chemistry Environmental Science The H-Sensor reported herein is a micro-fluidic device compatible with flow injection analysis (FIA) and high performance liquid chromatography (HPLC). The device detects analytes at two separate off-chip absorbance flow cells, providing two simultaneous absorbance measurements. The ratio of these two absorbance signals contains analyte diffusion coefficient information. A theoretical model for the sensing mechanism is presented. The model relates the signal Ratio to analyte diffusion coefficient. The model is qualitatively evaluated by comparing theoretical and experimental signal Ratio values. Experimental signal Ratios were collected via FIA for a variety of analytes, including sodium azide, benzoic acid, amino acids, peptides, and proteins. Measuring absorbance at multiple wavelengths provides higher order data allowing the analyte signals from mixtures to be deconvolved via classical least squares (CLS). As a result of the H-Sensor providing two simultaneous signals as a function of time for each sample injection, two simulated second-order HPLC chromatograms were generated using experimental H-Sensor data. The chemometric deconvolution method referred to as the generalized rank annihilation method (GRAM) was used to demonstrate chromatographic and spectroscopic deconvolution. GRAM also provides the signal Ratio value, therefore simultaneously obtaining the analyte diffusion coefficient information during deconvolution. The two chromatograms successfully serve as the standard and unknown for the GRAM deconvolution. GRAM was evaluated on chromatograms at various chromatographic resolutions. GRAM was found to function to a chromatographic resolution at and above 0.25 with a percent quantitative error of less then 10%. © 2006 Elsevier B.V. All rights reserved. 2018-09-11T08:54:26Z 2018-09-11T08:54:26Z 2006-08-11 Journal 00032670 2-s2.0-33746379878 10.1016/j.aca.2006.05.083 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33746379878&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/61511
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
Chemistry
Environmental Science
spellingShingle Biochemistry, Genetics and Molecular Biology
Chemistry
Environmental Science
Adam D. McBrady
Rattikan Chantiwas
Ana Kristine Torgerson
Kate Grudpan
Robert E. Synovec
An absorbance-based micro-fluidic sensor for diffusion coefficient and molar mass determinations
description The H-Sensor reported herein is a micro-fluidic device compatible with flow injection analysis (FIA) and high performance liquid chromatography (HPLC). The device detects analytes at two separate off-chip absorbance flow cells, providing two simultaneous absorbance measurements. The ratio of these two absorbance signals contains analyte diffusion coefficient information. A theoretical model for the sensing mechanism is presented. The model relates the signal Ratio to analyte diffusion coefficient. The model is qualitatively evaluated by comparing theoretical and experimental signal Ratio values. Experimental signal Ratios were collected via FIA for a variety of analytes, including sodium azide, benzoic acid, amino acids, peptides, and proteins. Measuring absorbance at multiple wavelengths provides higher order data allowing the analyte signals from mixtures to be deconvolved via classical least squares (CLS). As a result of the H-Sensor providing two simultaneous signals as a function of time for each sample injection, two simulated second-order HPLC chromatograms were generated using experimental H-Sensor data. The chemometric deconvolution method referred to as the generalized rank annihilation method (GRAM) was used to demonstrate chromatographic and spectroscopic deconvolution. GRAM also provides the signal Ratio value, therefore simultaneously obtaining the analyte diffusion coefficient information during deconvolution. The two chromatograms successfully serve as the standard and unknown for the GRAM deconvolution. GRAM was evaluated on chromatograms at various chromatographic resolutions. GRAM was found to function to a chromatographic resolution at and above 0.25 with a percent quantitative error of less then 10%. © 2006 Elsevier B.V. All rights reserved.
format Journal
author Adam D. McBrady
Rattikan Chantiwas
Ana Kristine Torgerson
Kate Grudpan
Robert E. Synovec
author_facet Adam D. McBrady
Rattikan Chantiwas
Ana Kristine Torgerson
Kate Grudpan
Robert E. Synovec
author_sort Adam D. McBrady
title An absorbance-based micro-fluidic sensor for diffusion coefficient and molar mass determinations
title_short An absorbance-based micro-fluidic sensor for diffusion coefficient and molar mass determinations
title_full An absorbance-based micro-fluidic sensor for diffusion coefficient and molar mass determinations
title_fullStr An absorbance-based micro-fluidic sensor for diffusion coefficient and molar mass determinations
title_full_unstemmed An absorbance-based micro-fluidic sensor for diffusion coefficient and molar mass determinations
title_sort absorbance-based micro-fluidic sensor for diffusion coefficient and molar mass determinations
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33746379878&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/61511
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