Improvement of myrosinase activity of Aspergillus sp. NR4617 by chemical mutagenesis

A myrosinase (thioglucoside glucohydrolase or thioglucosidase, EC 3.2.3.147) producing fungus, Aspergillus sp. NR4617, was newly isolated from decayed soil sample obtained in Thailand and was subjected to single exposure to two chemical mutagens, ethyl methanesulfonate (EMS) and N-methyl-N′- nitro-N...

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Main Authors: Nuansri Rakariyatham, Bordin Butr-Indr, Hataichanoke Niamsup, Lalida Shank
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/61513
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-615132018-09-11T08:57:45Z Improvement of myrosinase activity of Aspergillus sp. NR4617 by chemical mutagenesis Nuansri Rakariyatham Bordin Butr-Indr Hataichanoke Niamsup Lalida Shank Biochemistry, Genetics and Molecular Biology Immunology and Microbiology A myrosinase (thioglucoside glucohydrolase or thioglucosidase, EC 3.2.3.147) producing fungus, Aspergillus sp. NR4617, was newly isolated from decayed soil sample obtained in Thailand and was subjected to single exposure to two chemical mutagens, ethyl methanesulfonate (EMS) and N-methyl-N′- nitro-N-nitrosoguanidine (MNNG). Its myrosinase production was selected on low cost medium prepared from mustard seed cake (Brassica juncea). Studies of production and stability of the enzyme showed that EMS mutagenesis increased myrosinase activity. Aspergillus sp. NR4617E1 produced myrosinase 1.90 U ml -1 at 36 hrs of the cultivation equivalent to 171% of the enzyme production in wild-type. The stability studies revealed that myrosinase from the mutant strains retained activity similar to wild-type at 30°C. Aspergillus sp. NR4617E1 degraded 10 mM of glucosinolate completely in 36 hrs. Enhanced myrosinase production and high yields of products (allylisothiocyanate) demonstrated that this mutant could be a new found candidate for feed detoxification and industrial allylisothiocyanate production. © 2006 by Pontificia Universidad Católica de Valparaíso. 2018-09-11T08:54:27Z 2018-09-11T08:54:27Z 2006-07-15 Journal 07173458 07173458 2-s2.0-33747097967 10.2225/vol9-issue4-fulltext-13 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33747097967&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/61513
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Biochemistry, Genetics and Molecular Biology
Immunology and Microbiology
spellingShingle Biochemistry, Genetics and Molecular Biology
Immunology and Microbiology
Nuansri Rakariyatham
Bordin Butr-Indr
Hataichanoke Niamsup
Lalida Shank
Improvement of myrosinase activity of Aspergillus sp. NR4617 by chemical mutagenesis
description A myrosinase (thioglucoside glucohydrolase or thioglucosidase, EC 3.2.3.147) producing fungus, Aspergillus sp. NR4617, was newly isolated from decayed soil sample obtained in Thailand and was subjected to single exposure to two chemical mutagens, ethyl methanesulfonate (EMS) and N-methyl-N′- nitro-N-nitrosoguanidine (MNNG). Its myrosinase production was selected on low cost medium prepared from mustard seed cake (Brassica juncea). Studies of production and stability of the enzyme showed that EMS mutagenesis increased myrosinase activity. Aspergillus sp. NR4617E1 produced myrosinase 1.90 U ml -1 at 36 hrs of the cultivation equivalent to 171% of the enzyme production in wild-type. The stability studies revealed that myrosinase from the mutant strains retained activity similar to wild-type at 30°C. Aspergillus sp. NR4617E1 degraded 10 mM of glucosinolate completely in 36 hrs. Enhanced myrosinase production and high yields of products (allylisothiocyanate) demonstrated that this mutant could be a new found candidate for feed detoxification and industrial allylisothiocyanate production. © 2006 by Pontificia Universidad Católica de Valparaíso.
format Journal
author Nuansri Rakariyatham
Bordin Butr-Indr
Hataichanoke Niamsup
Lalida Shank
author_facet Nuansri Rakariyatham
Bordin Butr-Indr
Hataichanoke Niamsup
Lalida Shank
author_sort Nuansri Rakariyatham
title Improvement of myrosinase activity of Aspergillus sp. NR4617 by chemical mutagenesis
title_short Improvement of myrosinase activity of Aspergillus sp. NR4617 by chemical mutagenesis
title_full Improvement of myrosinase activity of Aspergillus sp. NR4617 by chemical mutagenesis
title_fullStr Improvement of myrosinase activity of Aspergillus sp. NR4617 by chemical mutagenesis
title_full_unstemmed Improvement of myrosinase activity of Aspergillus sp. NR4617 by chemical mutagenesis
title_sort improvement of myrosinase activity of aspergillus sp. nr4617 by chemical mutagenesis
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33747097967&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/61513
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