A simple manual rosetting method for absolute CD4⁺lymphocyte counting in resource-limited countries

• Main Authors: Phonethipsavanh Nouanthong, Supansa Pata, Thira Sirisanthana, Watchara Kasinrerk
• Format: Journal
• Published: 2018
• Subjects: Biochemistry, Genetics and Molecular Biology; Immunology and Microbiology; Medicine
• Online Access: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33646713920&origin=inward; http://cmuir.cmu.ac.th/jspui/handle/6653943832/61527
• Institution: Chiang Mai University

A CD4 monoclonal antibody which reacts with CD4+lymphocytes but does not react significantly with monocytes was generated and used to develop a simple method for CD4 count. In a comparison with standard flow cytometry, high correlation was obtained. The developed method can be an alternative to flow...