Pulsed amperometry for anti-fouling of boron-doped diamond in electroanalysis of β-agonists: Application to flow injection for pharmaceutical analysis

• Main Authors: Chanpen Karuwan, Thitirat Mantim, Patcharin Chaisuwan, Prapin Wilairat, Kate Grudpan, Piyada Jittangprasert, Yasuaki Einaga, Orawon Chailapakul, Leena Suntornsuk, Oraphan Anurukvorakun, Duangjai Nacapricha
• Format: Journal
• Published: 2018
• Subjects: Biochemistry, Genetics and Molecular Biology; Chemistry; Engineering; Physics and Astronomy
• Online Access: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33947367459&origin=inward; http://cmuir.cmu.ac.th/jspui/handle/6653943832/61556
• Institution: Chiang Mai University

This work presents the construction and application of boron-doped diamond (BDD) thin film electrode as sensor for the determination of three β-agonists, viz. salbutamol, terbutaline and clenbuterol. Although well-known as a chemically inert material, BDD film however shows fouling in detection of these compounds using fixed-potential mode amperometry. A suitable waveform for pulsed amperometric detection (PAD) was developed and used to determine the agonist compounds. It was seen that the developed PAD significantly refreshed the BDD surface for long-term detection in flow injection analysis. Linear working ranges were 0.5-100 μM, 1.0-100 μM and 0.5-50 μM for salbutamol, terbutaline and clenbuterol, respectively. The developed PAD-BDD system was applied to successfully determine salbutamol and terbutaline in commercial pharmaceutical products. The methods were validated with a capillary electrophoresis method. © 2006 by MDPI.