Determination of cefadroxil by sequential injection with spectrophotometric detector

A sequential injection analysis (SIA) spectrophotometric procedure for cefadroxil determination has been developed. The SIA instrumentation was modified to achieve the desired function and operations by using the software developed to interface the PC with the conventional SIA system. The method is...

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Main Authors: Jirayu Makchit, Suphachock Upalee, Chalermporn Thongpoon, Boonsom Liawruangrath, Saisunee Liawruangrath
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/61581
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-615812018-09-11T08:55:33Z Determination of cefadroxil by sequential injection with spectrophotometric detector Jirayu Makchit Suphachock Upalee Chalermporn Thongpoon Boonsom Liawruangrath Saisunee Liawruangrath Chemistry A sequential injection analysis (SIA) spectrophotometric procedure for cefadroxil determination has been developed. The SIA instrumentation was modified to achieve the desired function and operations by using the software developed to interface the PC with the conventional SIA system. The method is based on the measurement of a red, water-soluble product formed by the reaction between cefadroxil and 4-aminoantipyrine in the presence of alkaline potassium hexacyanoferrate(III) at 510 nm. Optimum conditions for determining the drug were investigated. Beer's law was obeyed over the concentration ranges of 1 - 10 mg L-1 and 10 - 50 mg L-1 with a detection limit (3σ) of 0.17 mg L-1 and a limit of quantification (10σ) of 0.56 mg L-1. The relative standard deviations of 1.98% and 1.93% for 5 mg L-1 and 30 mg L-1 of the drug, respectively (n = 11) are obtained. The proposed method has been applied satisfactorily to the determination of cefadroxil in commercial pharmaceutical formulations with a sampling rate of 100 h-1. Results obtained were in good agreement with those obtained by the official HPLC method at the 95% confidence level. 2006 © The Japan Society for Analytical Chemistry. 2018-09-11T08:55:33Z 2018-09-11T08:55:33Z 2006-04-01 Journal 13482246 09106340 2-s2.0-33646097910 10.2116/analsci.22.591 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33646097910&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/61581
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Chemistry
spellingShingle Chemistry
Jirayu Makchit
Suphachock Upalee
Chalermporn Thongpoon
Boonsom Liawruangrath
Saisunee Liawruangrath
Determination of cefadroxil by sequential injection with spectrophotometric detector
description A sequential injection analysis (SIA) spectrophotometric procedure for cefadroxil determination has been developed. The SIA instrumentation was modified to achieve the desired function and operations by using the software developed to interface the PC with the conventional SIA system. The method is based on the measurement of a red, water-soluble product formed by the reaction between cefadroxil and 4-aminoantipyrine in the presence of alkaline potassium hexacyanoferrate(III) at 510 nm. Optimum conditions for determining the drug were investigated. Beer's law was obeyed over the concentration ranges of 1 - 10 mg L-1 and 10 - 50 mg L-1 with a detection limit (3σ) of 0.17 mg L-1 and a limit of quantification (10σ) of 0.56 mg L-1. The relative standard deviations of 1.98% and 1.93% for 5 mg L-1 and 30 mg L-1 of the drug, respectively (n = 11) are obtained. The proposed method has been applied satisfactorily to the determination of cefadroxil in commercial pharmaceutical formulations with a sampling rate of 100 h-1. Results obtained were in good agreement with those obtained by the official HPLC method at the 95% confidence level. 2006 © The Japan Society for Analytical Chemistry.
format Journal
author Jirayu Makchit
Suphachock Upalee
Chalermporn Thongpoon
Boonsom Liawruangrath
Saisunee Liawruangrath
author_facet Jirayu Makchit
Suphachock Upalee
Chalermporn Thongpoon
Boonsom Liawruangrath
Saisunee Liawruangrath
author_sort Jirayu Makchit
title Determination of cefadroxil by sequential injection with spectrophotometric detector
title_short Determination of cefadroxil by sequential injection with spectrophotometric detector
title_full Determination of cefadroxil by sequential injection with spectrophotometric detector
title_fullStr Determination of cefadroxil by sequential injection with spectrophotometric detector
title_full_unstemmed Determination of cefadroxil by sequential injection with spectrophotometric detector
title_sort determination of cefadroxil by sequential injection with spectrophotometric detector
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33646097910&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/61581
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