The effect of Phyllanthus taxodiifolius beille extracts and its triterpenoids studying on cellular energetic stage of cancer cells

Problem statement: The Multidrug Resistance phenomenon (MDR) is the cause of unsuccessful in cancer chemotherapy. The tradition plant is the population used as the alternative medicine in cancer therapy. Due to, P. taxodiifolius is medicinal Thai plant which is used as diuretic drug which has never...

Full description

Saved in:
Bibliographic Details
Main Authors: Sakkrom P., Pompimon W., Meepowpan P., Nuntasaen N., Loetchutinat C.
Format: Article
Language:English
Published: 2014
Online Access:http://www.scopus.com/inward/record.url?eid=2-s2.0-79960405235&partnerID=40&md5=7793a26f0c3a959a6c451e036231633e
http://cmuir.cmu.ac.th/handle/6653943832/6166
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Chiang Mai University
Language: English
id th-cmuir.6653943832-6166
record_format dspace
spelling th-cmuir.6653943832-61662014-08-30T03:23:54Z The effect of Phyllanthus taxodiifolius beille extracts and its triterpenoids studying on cellular energetic stage of cancer cells Sakkrom P. Pompimon W. Meepowpan P. Nuntasaen N. Loetchutinat C. Problem statement: The Multidrug Resistance phenomenon (MDR) is the cause of unsuccessful in cancer chemotherapy. The tradition plant is the population used as the alternative medicine in cancer therapy. Due to, P. taxodiifolius is medicinal Thai plant which is used as diuretic drug which has never been explored as the anticancer activities. Approach: Air-dried powder of P. taxodiifolius leaves and twigs were serially extracted by hexane, ethyl acetate, acetone and methanol. These four extracts were tested the antiproliferative activity on four cancer cell lines. These results lead to successively purify two triternoids that are glochidone (1) and lupeol (2). Both pure compounds were tested the anticancer properties on same cancer cell lines and further investigate the cellular energetic state perturbation by measuring the mitochondrial membrane potential modification. Results: Four crude extracts were extracted and two triterpenoids (glochidone and lupeol) were purified and identified from hexane extract. Our antiproliferative activity of both compounds respectively showed in the IC 50 value of K562, K562/Adr, GLC4 and GLC4/Adr equal to 2.2±0.6, 4.2±1.5. 3.1±1.0 and 3.2±0.9 μg mL -1 for glochidone and 2.3±0.6, 4.5±1.7, 2.3±0.5 and 2.6±0.5 μg mL -1 for lupeol. The R value, which represents the multidrug resistance phenotype, is about 2 for P-glycloprotein overexpression in K562/Adr and 1 for MRP1 overexpression in GLC4/Adr. Conclusion: All crude extractions and two triterpenoids show the clear evidence of anticancer activity of both sensitive and resistance of erythroleukemic and Small cell lung cancer cell lines. Both compounds are not recognized by ABCB1 and ABCC1 proteins. Our results also indicated that lupeol initiate cell death by mitochondria membrane potential modification specially the sensitive cell line. © 2010 Science Publications. 2014-08-30T03:23:54Z 2014-08-30T03:23:54Z 2010 Article 15574962 10.3844/ajptsp.2010.139.144 http://www.scopus.com/inward/record.url?eid=2-s2.0-79960405235&partnerID=40&md5=7793a26f0c3a959a6c451e036231633e http://cmuir.cmu.ac.th/handle/6653943832/6166 English
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
language English
description Problem statement: The Multidrug Resistance phenomenon (MDR) is the cause of unsuccessful in cancer chemotherapy. The tradition plant is the population used as the alternative medicine in cancer therapy. Due to, P. taxodiifolius is medicinal Thai plant which is used as diuretic drug which has never been explored as the anticancer activities. Approach: Air-dried powder of P. taxodiifolius leaves and twigs were serially extracted by hexane, ethyl acetate, acetone and methanol. These four extracts were tested the antiproliferative activity on four cancer cell lines. These results lead to successively purify two triternoids that are glochidone (1) and lupeol (2). Both pure compounds were tested the anticancer properties on same cancer cell lines and further investigate the cellular energetic state perturbation by measuring the mitochondrial membrane potential modification. Results: Four crude extracts were extracted and two triterpenoids (glochidone and lupeol) were purified and identified from hexane extract. Our antiproliferative activity of both compounds respectively showed in the IC 50 value of K562, K562/Adr, GLC4 and GLC4/Adr equal to 2.2±0.6, 4.2±1.5. 3.1±1.0 and 3.2±0.9 μg mL -1 for glochidone and 2.3±0.6, 4.5±1.7, 2.3±0.5 and 2.6±0.5 μg mL -1 for lupeol. The R value, which represents the multidrug resistance phenotype, is about 2 for P-glycloprotein overexpression in K562/Adr and 1 for MRP1 overexpression in GLC4/Adr. Conclusion: All crude extractions and two triterpenoids show the clear evidence of anticancer activity of both sensitive and resistance of erythroleukemic and Small cell lung cancer cell lines. Both compounds are not recognized by ABCB1 and ABCC1 proteins. Our results also indicated that lupeol initiate cell death by mitochondria membrane potential modification specially the sensitive cell line. © 2010 Science Publications.
format Article
author Sakkrom P.
Pompimon W.
Meepowpan P.
Nuntasaen N.
Loetchutinat C.
spellingShingle Sakkrom P.
Pompimon W.
Meepowpan P.
Nuntasaen N.
Loetchutinat C.
The effect of Phyllanthus taxodiifolius beille extracts and its triterpenoids studying on cellular energetic stage of cancer cells
author_facet Sakkrom P.
Pompimon W.
Meepowpan P.
Nuntasaen N.
Loetchutinat C.
author_sort Sakkrom P.
title The effect of Phyllanthus taxodiifolius beille extracts and its triterpenoids studying on cellular energetic stage of cancer cells
title_short The effect of Phyllanthus taxodiifolius beille extracts and its triterpenoids studying on cellular energetic stage of cancer cells
title_full The effect of Phyllanthus taxodiifolius beille extracts and its triterpenoids studying on cellular energetic stage of cancer cells
title_fullStr The effect of Phyllanthus taxodiifolius beille extracts and its triterpenoids studying on cellular energetic stage of cancer cells
title_full_unstemmed The effect of Phyllanthus taxodiifolius beille extracts and its triterpenoids studying on cellular energetic stage of cancer cells
title_sort effect of phyllanthus taxodiifolius beille extracts and its triterpenoids studying on cellular energetic stage of cancer cells
publishDate 2014
url http://www.scopus.com/inward/record.url?eid=2-s2.0-79960405235&partnerID=40&md5=7793a26f0c3a959a6c451e036231633e
http://cmuir.cmu.ac.th/handle/6653943832/6166
_version_ 1681420562748932096