A biological evaluation of DNA damage detected by comet assay in healthy populations residing in areas that differ in lung cancer incidence

The comet assay was performed to evaluate the effect of environmental exposure between human populations residing in two areas that differ in lung cancer incidence, Saraphi ( n = 91) and Chom Thong ( n = 94). Three parameters, the tail length, tail intensity, and tail moment, were used to detect DNA...

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Main Authors: Worapa Heepchantree, Thipmani Paratasilpin, Daoroong Kangwanpong
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/61697
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spelling th-cmuir.6653943832-616972018-09-11T09:01:42Z A biological evaluation of DNA damage detected by comet assay in healthy populations residing in areas that differ in lung cancer incidence Worapa Heepchantree Thipmani Paratasilpin Daoroong Kangwanpong Environmental Science Pharmacology, Toxicology and Pharmaceutics The comet assay was performed to evaluate the effect of environmental exposure between human populations residing in two areas that differ in lung cancer incidence, Saraphi ( n = 91) and Chom Thong ( n = 94). Three parameters, the tail length, tail intensity, and tail moment, were used to detect DNA damage in peripheral blood and stimulated lymphocytes with and without the DNA repair inhibitor, aphidicolin. Internal standards, cryopreserved isolated lymphocytes, and isolated lymphocytes irradiated with 2 Gy gamma rays, were used to correct the interexperimental variability. Results revealed a significant difference between two populations only when the tail length was used to measure DNA damage. The evaluation of various potential confounding factors, such as gender, pesticide exposure, smoking, alcohol drinking, and fermented tea leaf or betel nut chewing, indicated no significant influence in DNA damage. In conclusion, significant difference in DNA damage, detected only by tail length between the two populations residing in the areas with different incidence of lung cancer, may reflect a nonhazardous level of exposure to toxic substances. Copyright© Taylor & Francis Group, LLC. 2018-09-11T08:57:17Z 2018-09-11T08:57:17Z 2006-06-01 Journal 10872620 15287394 2-s2.0-33745686443 10.1080/15287390500360257 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33745686443&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/61697
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Environmental Science
Pharmacology, Toxicology and Pharmaceutics
spellingShingle Environmental Science
Pharmacology, Toxicology and Pharmaceutics
Worapa Heepchantree
Thipmani Paratasilpin
Daoroong Kangwanpong
A biological evaluation of DNA damage detected by comet assay in healthy populations residing in areas that differ in lung cancer incidence
description The comet assay was performed to evaluate the effect of environmental exposure between human populations residing in two areas that differ in lung cancer incidence, Saraphi ( n = 91) and Chom Thong ( n = 94). Three parameters, the tail length, tail intensity, and tail moment, were used to detect DNA damage in peripheral blood and stimulated lymphocytes with and without the DNA repair inhibitor, aphidicolin. Internal standards, cryopreserved isolated lymphocytes, and isolated lymphocytes irradiated with 2 Gy gamma rays, were used to correct the interexperimental variability. Results revealed a significant difference between two populations only when the tail length was used to measure DNA damage. The evaluation of various potential confounding factors, such as gender, pesticide exposure, smoking, alcohol drinking, and fermented tea leaf or betel nut chewing, indicated no significant influence in DNA damage. In conclusion, significant difference in DNA damage, detected only by tail length between the two populations residing in the areas with different incidence of lung cancer, may reflect a nonhazardous level of exposure to toxic substances. Copyright© Taylor & Francis Group, LLC.
format Journal
author Worapa Heepchantree
Thipmani Paratasilpin
Daoroong Kangwanpong
author_facet Worapa Heepchantree
Thipmani Paratasilpin
Daoroong Kangwanpong
author_sort Worapa Heepchantree
title A biological evaluation of DNA damage detected by comet assay in healthy populations residing in areas that differ in lung cancer incidence
title_short A biological evaluation of DNA damage detected by comet assay in healthy populations residing in areas that differ in lung cancer incidence
title_full A biological evaluation of DNA damage detected by comet assay in healthy populations residing in areas that differ in lung cancer incidence
title_fullStr A biological evaluation of DNA damage detected by comet assay in healthy populations residing in areas that differ in lung cancer incidence
title_full_unstemmed A biological evaluation of DNA damage detected by comet assay in healthy populations residing in areas that differ in lung cancer incidence
title_sort biological evaluation of dna damage detected by comet assay in healthy populations residing in areas that differ in lung cancer incidence
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33745686443&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/61697
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