Binding of multivalent CD147 phage induces apoptosis of U937 cells

CD147 is a broadly expressed cell-surface molecule and serves as a signaling receptor for extracellular cyclophilins. CD147 also appears to interact with immune cells, but its counter-receptor on these cells has not been clearly described. In the present report, we displayed multiple copies of the C...

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Main Authors: Nutjeera Intasai, Sabine Mai, Watchara Kasinrerk, Chatchai Tayapiwatana
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/61715
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-617152018-09-11T08:57:49Z Binding of multivalent CD147 phage induces apoptosis of U937 cells Nutjeera Intasai Sabine Mai Watchara Kasinrerk Chatchai Tayapiwatana Immunology and Microbiology CD147 is a broadly expressed cell-surface molecule and serves as a signaling receptor for extracellular cyclophilins. CD147 also appears to interact with immune cells, but its counter-receptor on these cells has not been clearly described. In the present report, we displayed multiple copies of the CD147 extracellular domain (CD147Ex) on VCSM13 phage to study the interaction of CD147 with its ligand. Recognition of phage containing fusion protein of CD147Ex and gpVIII (CD147Ex phage) by four different anti-CD147 mAbs indicated that at least parts of the CD147 are properly folded. Specific binding of CD147Ex phage to various cell types was demonstrated by flow cytometry. Morphological changes, however, were observed only in U937, a monocytic cell line, after 24 h incubation with multivalent CD147Ex phage. After 48 h, U937 cell propagation ceased. Staining with annexin V and the presence of cleaved caspase-3 indicated that many of the CD147Ex phage-treated cells had lost viability through apoptotic cell death. The above results suggest that CD147 induces apoptosis in U973 cells and that at least a portion of this cell death program involves a caspase-dependent pathway. © 2006 Oxford University Press. 2018-09-11T08:57:49Z 2018-09-11T08:57:49Z 2006-07-01 Journal 14602377 09538178 2-s2.0-33745238125 10.1093/intimm/dxl050 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33745238125&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/61715
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Immunology and Microbiology
spellingShingle Immunology and Microbiology
Nutjeera Intasai
Sabine Mai
Watchara Kasinrerk
Chatchai Tayapiwatana
Binding of multivalent CD147 phage induces apoptosis of U937 cells
description CD147 is a broadly expressed cell-surface molecule and serves as a signaling receptor for extracellular cyclophilins. CD147 also appears to interact with immune cells, but its counter-receptor on these cells has not been clearly described. In the present report, we displayed multiple copies of the CD147 extracellular domain (CD147Ex) on VCSM13 phage to study the interaction of CD147 with its ligand. Recognition of phage containing fusion protein of CD147Ex and gpVIII (CD147Ex phage) by four different anti-CD147 mAbs indicated that at least parts of the CD147 are properly folded. Specific binding of CD147Ex phage to various cell types was demonstrated by flow cytometry. Morphological changes, however, were observed only in U937, a monocytic cell line, after 24 h incubation with multivalent CD147Ex phage. After 48 h, U937 cell propagation ceased. Staining with annexin V and the presence of cleaved caspase-3 indicated that many of the CD147Ex phage-treated cells had lost viability through apoptotic cell death. The above results suggest that CD147 induces apoptosis in U973 cells and that at least a portion of this cell death program involves a caspase-dependent pathway. © 2006 Oxford University Press.
format Journal
author Nutjeera Intasai
Sabine Mai
Watchara Kasinrerk
Chatchai Tayapiwatana
author_facet Nutjeera Intasai
Sabine Mai
Watchara Kasinrerk
Chatchai Tayapiwatana
author_sort Nutjeera Intasai
title Binding of multivalent CD147 phage induces apoptosis of U937 cells
title_short Binding of multivalent CD147 phage induces apoptosis of U937 cells
title_full Binding of multivalent CD147 phage induces apoptosis of U937 cells
title_fullStr Binding of multivalent CD147 phage induces apoptosis of U937 cells
title_full_unstemmed Binding of multivalent CD147 phage induces apoptosis of U937 cells
title_sort binding of multivalent cd147 phage induces apoptosis of u937 cells
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33745238125&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/61715
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