CD147 contains different bioactive epitopes involving the regulation of cell adhesion and lymphocyte activation

CD147 is a leukocyte surface molecule which belongs to the immunoglobulin superfamily. It is broadly expressed on various cell types and is a lymphocyte activation-associated molecule. In order to study the function of CD147, five CD147 monoclonal antibodies (mAbs) were generated: M6-2F9; M6-1D4; M6...

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Main Authors: Sawitree Chiampanichayakul, Pakorn Peng-in, Panida Khunkaewla, Hannes Stockinger, Watchara Kasinrerk
Format: Journal
Published: 2018
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http://cmuir.cmu.ac.th/jspui/handle/6653943832/61722
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Institution: Chiang Mai University
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spelling th-cmuir.6653943832-617222018-09-11T08:57:55Z CD147 contains different bioactive epitopes involving the regulation of cell adhesion and lymphocyte activation Sawitree Chiampanichayakul Pakorn Peng-in Panida Khunkaewla Hannes Stockinger Watchara Kasinrerk Immunology and Microbiology CD147 is a leukocyte surface molecule which belongs to the immunoglobulin superfamily. It is broadly expressed on various cell types and is a lymphocyte activation-associated molecule. In order to study the function of CD147, five CD147 monoclonal antibodies (mAbs) were generated: M6-2F9; M6-1D4; M6-1F3; M6-1B9; and M6-1E9. Biochemical characterizations and cross-blocking experiments indicated that M6-1B9 and M6-1E9 recognize the same or contiguous epitopes on CD147. By employing COS transfectants expressing CD147 membrane-distal domain (domain 1) and membrane-proximal domain (domain 2), mAbs M6-2F9, M6-1D4, M6-1B9, and M6-1E9 were shown to recognize epitopes located on domain 1 of the molecule. Functional studies indicated that engagement of CD147 by mAbs M6-1B9 and M6-1E9 strongly inhibited lymphocyte proliferation induced by a CD3 mAb. In contrast, mAbs M6-2F9, M6-1D4, and M6-1F3 induced U937 homotypic cell aggregation. The results indicate that CD147 contains at least two bioactive domains. Epitopes responsible for induction of cell aggregation are different from those regulating lymphocyte activation. © Elsevier GmbH. All rights reserved. 2018-09-11T08:57:55Z 2018-09-11T08:57:55Z 2006-04-03 Journal 01712985 2-s2.0-33644783638 10.1016/j.imbio.2005.08.007 https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33644783638&origin=inward http://cmuir.cmu.ac.th/jspui/handle/6653943832/61722
institution Chiang Mai University
building Chiang Mai University Library
country Thailand
collection CMU Intellectual Repository
topic Immunology and Microbiology
spellingShingle Immunology and Microbiology
Sawitree Chiampanichayakul
Pakorn Peng-in
Panida Khunkaewla
Hannes Stockinger
Watchara Kasinrerk
CD147 contains different bioactive epitopes involving the regulation of cell adhesion and lymphocyte activation
description CD147 is a leukocyte surface molecule which belongs to the immunoglobulin superfamily. It is broadly expressed on various cell types and is a lymphocyte activation-associated molecule. In order to study the function of CD147, five CD147 monoclonal antibodies (mAbs) were generated: M6-2F9; M6-1D4; M6-1F3; M6-1B9; and M6-1E9. Biochemical characterizations and cross-blocking experiments indicated that M6-1B9 and M6-1E9 recognize the same or contiguous epitopes on CD147. By employing COS transfectants expressing CD147 membrane-distal domain (domain 1) and membrane-proximal domain (domain 2), mAbs M6-2F9, M6-1D4, M6-1B9, and M6-1E9 were shown to recognize epitopes located on domain 1 of the molecule. Functional studies indicated that engagement of CD147 by mAbs M6-1B9 and M6-1E9 strongly inhibited lymphocyte proliferation induced by a CD3 mAb. In contrast, mAbs M6-2F9, M6-1D4, and M6-1F3 induced U937 homotypic cell aggregation. The results indicate that CD147 contains at least two bioactive domains. Epitopes responsible for induction of cell aggregation are different from those regulating lymphocyte activation. © Elsevier GmbH. All rights reserved.
format Journal
author Sawitree Chiampanichayakul
Pakorn Peng-in
Panida Khunkaewla
Hannes Stockinger
Watchara Kasinrerk
author_facet Sawitree Chiampanichayakul
Pakorn Peng-in
Panida Khunkaewla
Hannes Stockinger
Watchara Kasinrerk
author_sort Sawitree Chiampanichayakul
title CD147 contains different bioactive epitopes involving the regulation of cell adhesion and lymphocyte activation
title_short CD147 contains different bioactive epitopes involving the regulation of cell adhesion and lymphocyte activation
title_full CD147 contains different bioactive epitopes involving the regulation of cell adhesion and lymphocyte activation
title_fullStr CD147 contains different bioactive epitopes involving the regulation of cell adhesion and lymphocyte activation
title_full_unstemmed CD147 contains different bioactive epitopes involving the regulation of cell adhesion and lymphocyte activation
title_sort cd147 contains different bioactive epitopes involving the regulation of cell adhesion and lymphocyte activation
publishDate 2018
url https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=33644783638&origin=inward
http://cmuir.cmu.ac.th/jspui/handle/6653943832/61722
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